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Controllable formation of polydopamine on carbon dots for ultrasensitive detection of alkaline phosphatase and ratiometric fluorescence immunoassay of benzocaine

文献类型： 外文期刊

作者： Lin, Shi-Qi 1 ; Jia, Bao-Zhu 3 ; Luo, Wei 4 ; Wang, Hong 1 ; Lei, Hong-Tao 1 ; Zhang, Wen-Feng 2 ; Xu, Zhen-Lin 1 ; Luo, Lin 1 ;

作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

2.Guangdong Acad Sci, Guangdong Prov Engn Res Ctr Rapid Testing Instrume, Inst Anal, China Natl Analyt Ctr,Guangdong Prov Key Lab Chem, Guangzhou 510070, Peoples R China

3.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Peoples R China

4.Guangdong Acad Agr Sci, Inst Anim Sci, State Key Lab Livestock & Poultry Breeding, Guangzhou 510640, Peoples R China

5.Guangdong Acad Agr Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China

关键词： Alkaline phosphatase; Benzocaine; Ratiometric fluorescence; Immunoassay; Polydopamine; Carbon dots

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 426 卷

页码：

收录情况： SCI

摘要： Sensing alkaline phosphatase (ALP) activity with high sensitivity and accuracy is critical for both ALP-related health and food safety supervision and the development of ALP-triggered immunoassay platforms. Herein, an ultrasensitive ratiometric fluorescence (RF) sensing system based on the controllable formation of luminescent polydopamine and efficient quenching of carbon dots was proposed for the ALP activity assay, achieving quantitative detection in the range of 0.01-100 mU/L. Furthermore, this RF sensing system was integrated with an ALP-based ELISA platform to construct an RF-ELISA for benzocaine, a potentially abused anesthetic in edible fish, and ultrasensitive assay at the level of fg/mL was realized. This ratiometric strategy-based platform effectively shields various interferences through the self-calibration effect, thus providing more accurate and reliable quantification results. This study not only offers an efficient method for ultratrace detection of ALP and benzocaine but also proposes a universal platform for ultrasensitive detection of diverse targets in food analysis by replacing the recognition unit.

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