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Supercharged DNA origami enhanced signal amplification for ultrasensitive detection of nucleic acid

文献类型： 外文期刊

作者： Qin, Weiwei 1 ; Gong, Xueyan 1 ; Duan, Xueyuan 1 ; Hao, Jicong 1 ; Ren, Haiying 3 ; Shentu, Xuping 1 ; Ye, Zihong 1 ; Yu, Xiaoping 1 ;

作者机构： 1.China Jiliang Univ, Coll Life Sci, Key Lab Microbiol Metrol Measurement & Bioprod Qua, State Adm Market Regulat, Hangzhou 310018, Peoples R China

2.Air Force Med Ctr, Pharm Dept, Beijing 100142, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China

关键词： DNA origami; Signal amplification; Electrochemical sensor; Nucleic acid; Gold nanostructures; Peptide nucleic acids

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.5； 五年影响因子：10.1 ）

ISSN： 0956-5663

年卷期： 2025 年 287 卷

页码：

收录情况： SCI

摘要： DNA origami has been able to controllably construct highly precise and complex two-or three-dimensional nanostructures of various morphologies. However, their application in the biosensing field is still relatively limited. In this paper, we proposed a portable electrochemical sensor for the detection of nucleic acid targets, using circulating tumor DNA (ctDNA) as an example. This biosensor combined DNA origami with high-curvature gold nanostructures. First, the target was captured by DNA origami in a homogeneous solution and formed the DNA origami-ctDNA (DOC) complex. Subsequently, the DOC complex was further brought to the electrode surface through hybridization with peptide nucleic acids (PNAs) modified on the surface of the gold nano-structures. The high-curvature nanostructured surface reduces entanglement and aggregation between the PNA probes, enhancing their accessibility to DOC. Furthermore, the substantial intrinsic charge of DNA origami can adsorb numerous electroactive [Ru(NH3)6]3+, whereas PNAs are electrically neutral, significantly enhancing the detection sensitivity and reducing background signals, enabling ultrasensitive detection sensitivity. Additionally, we demonstrated that the DNA origami-based sensor enhanced signal amplification efficiency compared to single-stranded DNA and tetrahedral DNA nanostructures. Through this signal amplification strategy, the sensor achieves highly sensitive (0.26 fM), specific, and selective (in serum) detection of ctDNA, providing a new avenue for nonenzymatic signal amplification and expanding the application of DNA origami in the sensing field.

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