Identification of Sex-Specific Markers and Candidate Genes Using WGS Sequencing Reveals a ZW-Type Sex-Determination System in the Chinese Soft-Shell Turtle (Pelodiscus sinensis)
文献类型: 外文期刊
作者: Zhu, Junxian 1 ; Wang, Yongchang 2 ; Chen, Chen 2 ; Ji, Liqin 2 ; Hong, Xiaoyou 2 ; Liu, Xiaoli 2 ; Chen, Haigang 2 ; Wei, Chengqing 2 ; Zhu, Xinping 1 ; Li, Wei 1 ;
作者机构: 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China
关键词: Pelodiscus sinensis; whole-genome sequencing; sex-specific sequence screening; sex-specific markers; sex-determining candidate genes
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.6; 五年影响因子:6.2 )
ISSN: 1661-6596
年卷期: 2024 年 25 卷 2 期
页码:
收录情况: SCI
摘要: Male and female Chinese soft-shelled turtles (Pelodiscus sinensis) have sex-dimorphic growth patterns, and males have higher commercial value because of their larger size and thicker calipash. Thus, developing sex-specific markers is beneficial to studies on all-male breeding in P. sinensis. Here, we developed an accurate and efficient workflow for the screening of sex-specific sequences with ZW or XY sex determination systems. Based on this workflow, female and male P. sinensis reference genomes of 2.23 Gb and 2.26 Gb were obtained using de novo assembly. After aligning and filtering, 4.01 Mb female-specific sequences were finally identified. Subsequently, the seven developed sex-specific primer pairs were 100% accurate in preliminary, population, and embryonic validation. The presence and absence of bands for the primers of P44, P45, P66, P67, P68, and P69, as well as two and one bands for the PB1 primer, indicate that the embryos are genetically female and male, respectively. NR and functional annotations identified several sex-determining candidate genes and related pathways, including Ran, Eif4et, and Crkl genes, and the insulin signaling pathway and the cAMP signaling pathway, respectively. Collectively, our results reveal that a ZW-type sex-determination system is present in P. sinensis and provide novel insights for the screening of sex-specific markers, sex-control breeding, and the studies of the sex determination mechanism of P. sinensis.
- 相关文献
作者其他论文 更多>>
-
Assessing and Screening of Female Fertility in Artificially Bred Asian Yellow Pond Turtles (Mauremys mutica) Based on Parentage Assignment
作者:Zhang, Xincheng;Zhao, Jian;Zhou, Chenyao;Li, Wei;Liu, Yihui;Wei, Chengqing;Wang, Yakun;Hong, Xiaoyou;Zhu, Xinping;Liu, Xiaoli;Zhou, Chenyao
关键词:female fecundity; Mauremys mutica; microsatellite; breeder contributions; genetic variability
-
Distribution and typologies of anthropogenic seafloor litter in the Pearl River Estuary and adjacent coastal waters, China
作者:He, Mingqiao;He, Mingqiao;Tian, Fei;Zhang, Linbao;Tang, Zhenzhao;Chen, Haigang;Zhai, Xiaohui;Zhou, Kai;Guo, Xiangyu
关键词:Marine pollution; Plastic waste; Pearl River Estuary; Seafloor litter
-
Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection
作者:Ji, Liqin;Chen, Chen;Zhu, Junxian;Hong, Xiaoyou;Liu, Xiaoli;Wei, Chengqing;Zhu, Xinping;Li, Wei
关键词:Chinese soft-shelled turtle; plasma parameters; transcriptome; metabolome; liver; Aeromonas hydrophila
-
Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica)
作者:Liu, Xiaoli;Xu, Haoyang;Peng, Mingwei;Zhou, Chenyao;Wei, Chengqing;Hong, Xiaoyou;Li, Wei;Chen, Chen;Ji, Liqin;Zhu, Xinping;Liu, Xiaoli;Xu, Haoyang;Li, Wei;Zhu, Xinping;Peng, Mingwei;Zhou, Chenyao;Zhu, Xinping
关键词:Turtle; Gonadal transcriptome; RNA-seq; Sexual dimorphism; Temperature-dependent sex determination
-
Development and characterization of 68 SNPs markers in the critically endangered species, Palea steindachneri
作者:Zhu, Junxian;Liu, Pan;Ji, Liqin;Hong, Xiaoyou;Liu, Xiaoli;Chen, Haigang;Wei, Chengqing;Zhu, Xinping;Chen, Chen;Li, Wei;Zhu, Junxian;Zhu, Xinping;Li, Wei
关键词:Palea steindachneri; Critically endangered species; Genetic resources conservation; SNP marker
-
Effects of Photoperiod on Survival, Growth, Physiological, and Biochemical Indices of Redclaw Crayfish (Cherax quadricarinatus) Juveniles
作者:Nie, Xiangxing;Huang, Cuixue;Chu, Zhangjie;Nie, Xiangxing;Huang, Cuixue;Wei, Jie;Wang, Yakun;Hong, Kunhao;Zhu, Xinping;Yu, Lingyun;Mu, Xidong;Liu, Chao
关键词:aquaculture; circulating water culture; environmental factor; body color; gene expression
-
Comparative genomic survey and functional analysis of DKKL1 during spermatogenesis in the Chinese soft-shelled turtle (Pelodiscus sinensis)
作者:Zhu, Junxian;Wang, Yongchang;Lei, Luo;Chen, Chen;Ji, Liqin;Wu, Congcong;Yu, Wenjun;Luo, Laifu;Chen, Weiqin;Liu, Pan;Hong, Xiaoyou;Liu, Xiaoli;Chen, Haigang;Wei, Chengqing;Zhu, Xinping;Li, Wei;Zhu, Junxian;Zhu, Xinping;Wang, Yongchang;Li, Jiansong
关键词:Pelodiscus sinensis; DKKL1; Spermatogenesis; Comparative genomics analysis; Functional validation