文献类型： 外文期刊

作者： Shao, Liu ¹ ; Chen, Shuqin ¹ ; Liu, Jiaxiang ¹ ; Yao, Xinjie ¹ ; Zhou, Xiaolong ¹ ; He, Yongqing ¹ ; Ju, Yutong ² ; Li, Tianyu ¹ ; Yan, Tingting ³ ; Xiao, Kun ⁴ ; Mo, Weiping ⁵ ; Wang, Lin ¹ ;

作者机构： 1.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China

2.China Agr Univ, Sanya Inst, Sanya 572025, Hainan, Peoples R China

3.Hainan Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Genet Resources Evaluat & Utilizat Trop Fr, Inst Trop Fruit Trees,Key Lab Trop Fruit Tree Biol, Haikou 571100, Hainan, Peoples R China

4.Hebei Normal Univ Sci & Technol, Coll Hort Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China

5.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100093, Peoples R China

关键词： Post-translational modification; MdZTL1; MdCOMT1

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 319 卷

页码：

收录情况： SCI

摘要： Caffeic acid O-methyltransferase (COMT) is involved in melatonin biosynthesis and regulates salt tolerance in plants, but no COMT homologs have been reported in apple. The post-translational mechanisms through which COMT regulates salt tolerance remain elusive in apple. In this study, we identified a novel protein module MdZTL1-MdCOMT1 regulating melatonin biosynthesis and salt tolerance. MdCOMT1 was a key bioactive melatonin synthetase and exhibited positive biology activity in enhancing salt tolerance of apple. MdCOMT1 could interact with MdZTL1, a F-box ubiquitin-ligating enzyme (E3). Interestingly, MdZTL1 negatively regulated melatonin biosynthesis and salt tolerance by forming the multimeric E3 complex SCFZTL1 to target the ubiquitination of MdCOMT1 and its subsequent degradation in the 26S proteasome. These findings suggest that MdZTL1 inhibits the promotion of MdCOMT1 in salt tolerance, with decreasing melatonin biosynthesis and impairing subsequent ROS scavenging. Our study reveals a ubiquitination-mediated post-translational regulation mechanism in salt tolerance, providing novel insights into the improvement of salt-tolerant apple through molecular design breeding.