您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Potency and efficacy of VP20-based vaccine against tilapia lake virus using different prime-boost vaccination regimens in tilapia

文献类型： 外文期刊

作者： Zeng, Weiwei 1 ; Wang, Yingying 2 ; Chen, Xiaoyu 4 ; Wang, Qing 2 ; Bergmann, Sven M. 3 ; Yang, Ying 1 ; Wang, Yahui 2 ;

作者机构： 1.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr,Key Lab Aquat Anim Immune Technol, Guangzhou 510380, Guangdong, Peoples R China

3.Friedrich Loffler Inst FLI, Fed Res Inst Anim Hlth, Inst Infectol, Greifswald, Germany

4.Technol Ctr Wuhan Customs, Wuhan 430050, Hubei, Peoples R China

5.Shenzhen Acad Sci & Technol Inspect & Quarantine, Shenzhen R&D Key Lab Alien Pest Detect Technol, Technol Ctr Anim & Plant Inspect & Quarantine, Shenzhen Customs, Shenzhen 518010, Peoples R China

关键词： Tilapia lake virus; VP20; DNA vaccine; Protein vaccine; Prime-boost

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2021 年 539 卷

页码：

收录情况： SCI

摘要： Tilapia lake virus (TiLV) is a newly emerged pathogen responsible for high mortality and economic losses in the global tilapia industry. Vaccination is the most effective way to prevent and control viral diseases. There is currently no vaccine on the market to control TiLV infections. Recently, we identified that TiLV segment 8 encoded a protein (VP20) with immunogenicity sufficient for use as a vaccine antigen. In this study, the immune responses and protective efficacy elicited by VP20 with different prime-boost vaccination regimens (DNA only, protein only, and DNA plus protein) in tilapia was evaluated. The results indicated that both pV-optiVP20 plasmid and rVP20 protein induced humoral and cellular immune responses. Tilapia in the DNA primeprotein boost group developed significantly higher levels of antibody response compared with those immunized with either the DNA or the protein alone (P < 0.05). Furthermore, the highest mRNA levels of the genes TNF-?, IL-1?, IgM, CD4, MHC-Ia and MHC-II were induced following priming with the pV-optiVP20 and boosting with the rVP20. Additionally, tilapia inoculated with the pV-optiVP20 prime-rVP20 boost regimen had a 72.5% survival rate against challenge with the lethal TiLV 2017A compared with 50% or 52.5% survival using the pVoptiVP20 and the rVP20 vaccinations alone. These findings demonstrated that VP20-based vaccine could elicit both humoral and cellular immune responses and significantly protected the fish against infectious challenge by TiLV. The DNA prime-protein boost immunization strategy showed the potential advantage over a solo vaccination.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台