A novel salt-tolerant GH42 beta-galactosidase with transglycosylation activity from deep-sea metagenome
文献类型： 外文期刊
作者： Sun, Jingjing 1 ; Yao, Congyu 1 ; Li, Yujie 4 ; Wang, Wei 1 ; Hao, Jianhua 1 ; Yu, Yi 6 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266000, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266000, Peoples R China
3.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214000, Jiangsu, Peoples R China
4.Shandong Univ Sci & Technol, Coll Safety & Environm Engn, Qingdao 266000, Peoples R China
5.Jiangsu Collaborat Innovat Ctr Exploitat & Utiliz, Lianyungang 222000, Peoples R China
6.Qingdao Univ, Affiliated Hosp, Qingdao 266000, Peoples R China
关键词： beta-Galactosidase; Metagenome; Lactose; Transglycosylation; Salt tolerance
期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.253； 五年影响因子：4.272 ）
ISSN： 0959-3993
年卷期： 2022 年 38 卷 9 期
页码：
收录情况： SCI
摘要： beta-Galactosidase is a widely adopted enzyme in the food and pharmaceutical industries. Metagenome techniques have the advantage of discovering novel functional genes, particularly potential genes from uncultivated microbes. In this study, a novel GH42 beta-galactosidase isolated from a deep-sea metagenome was overexpressed in Escherichia coli BL21 (DE3) and purified by affinity chromatography. The optimal temperatures and pH of the enzyme for o-nitrophenyl-beta-D-galactopyranoside (oNPG) and lactose were 40 degrees C, 6.5 and 50 degrees C, 7, respectively. The enzyme was stable at temperatures between 4 and 30 degrees C and within the pH range of 6-9. Moreover, it was highly tolerant to salt and inhibited by Zn2+ and Cu2+. The kinetic values of K-m and k(cat) of the enzyme against oNPG were 1.1 mM and 57.8 s(-1), respectively. Furthermore, it showed hydrolysis and transglycosylation activity to lactose and the extra monosaccharides could improve the productivity of oligosaccharides. Overall, this recombinant beta-galactosidase is a potential biocatalyst for the hydrolysis of milk lactose and synthesis of functional oligosaccharides.
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