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Dendrobium nobile active ingredient Dendrobin A against hepatocellular carcinoma via inhibiting nuclear factor kappa-B signaling

文献类型： 外文期刊

作者： Yu, Yaping 1 ; Fan, Yonghao 1 ; Mei, Wenli 3 ; Xu, Xiaoqing 1 ; Chen, Yan 1 ; Zhao, Yangyang 1 ; Ruan, Banzhan 1 ; Shen, Zhihua 4 ; Lu, Yanda 1 ; Zheng, Shaojiang 1 ; Jie, Wei 1 ;

作者机构： 1.Hainan Med Univ, Affiliated Hosp 1, Engn Res Ctr Hainan Biol Sample Resources Major Di, Key Lab Emergency & Trauma,Minist Educ, Haikou 570102, Peoples R China

2.Hainan Med Univ, Dept Oncol Affiliated Hosp 1, Haikou 570102, Peoples R China

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Nat Prod Res & Dev Li Folk Med Hainan Prov, Haikou 571199, Peoples R China

4.Guangdong Med Univ, Sch Basic Med Sci, Dept Pathophysiol, Zhanjiang 524023, Peoples R China

关键词： Hepatocellular carcinoma; Dendrobin A; Biological function; mRNA sequencing; NF-kappa B pathway

期刊名称：BIOMEDICINE & PHARMACOTHERAPY （ 影响因子：7.5； 五年影响因子：7.7 ）

ISSN： 0753-3322

年卷期： 2024 年 177 卷

页码：

收录情况： SCI

摘要： Objective: Dendrobin A, a typical active ingredient of the traditional Chinese medicine Dendrobium nobile, , potential clinical application in cancer treatment; however, its effect and mechanism in anti-hepatocellular carcinoma (HCC) remain unsolved. Method: The effects of Dendrobin A on the viability, migration, invasion, cycle, apoptosis, and epithelialmesenchymal transition of HepG2 and SK-HEP-1 cells were verified by in vitro experiments. mRNA sequencing was performed to screen the differentially expressed genes (DEGs) of HCC cells before and after Dendrobin treatment, following GO enrichment and KEGG signaling pathway analyses. Mechanistically, molecular docking was used to evaluate the binding of Dendrobin A with proteins p65 and p50, before further verifying the vation of nuclear factor kappa-B (NF-kappa B) kappa B) signaling. Finally, the antiproliferative effect of Dendrobin A on cells was explored through animal experiments. Results: Dendrobin A arrested cell cycle, induced apoptosis, and inhibited proliferation, migration, invasion, blocked epithelial-mesenchymal transition in HepG2 and SK-HEP-1 cells. mRNA sequencing identified 830 DEGs, involving various biological processes. KEGG analysis highlighted NF-kappa B kappa B signaling. Molecular docking revealed strong binding of Dendrobin A with p65 and p50 proteins, and western blotting confirmed reduced levels p65 and p-p50 in HCC cells post Dendrobin A treatment. NF-kappa B kappa B agonist PMA reversed Dendrobin A-inhibited proliferation migration and invasion. In vivo experiments showed that Dendrobin A inhibited HCC cell growth. Conclusion: Our findings suggest that Dendrobin A exhibits anti-HCC properties by inhibiting the activation of NF-kappa B kappa B pathway. These results provide a scientific basis for utilizing Dendrobium nobile in anti-HCC therapies.

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