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Molecular evolution and characterization of type III polyketide synthase gene family in Aquilaria sinensis

文献类型： 外文期刊

作者： Ding, Xupo 1 ; Wang, Hao 1 ; Huang, Shengzhou 1 ; Zhang, Hao 1 ; Chen, Huiqin 1 ; Chen, Pengwei 1 ; Wang, Yuguang 2 ; Yang, Zhuo 1 ; Wang, Yali 2 ; Peng, Shiqing 1 ; Dai, Haofu 1 ; Mei, Wenli 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Res & Dev Nat Prod Li Folk Med Hainan Prov, Haikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Int Joint Res Ctr Agarwood, Haikou 571101, Peoples R China

3.Inst Trop Biosci & Biotechnol, Chinese Acad Trop Agr Sci, Hainan Engn Res Ctr Agarwood, Haikou 571101, Peoples R China

4.Inst Trop Biosci & Biotechnol, Chinese Acad Trop Agr Sci, Haikou, Peoples R China

关键词： Polyketide synthase; 2-(2-Phenylethyl) chromone biosynthesis; Agarwood; Evolution; Transient expression

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2024 年 210 卷

页码：

收录情况： SCI

摘要： 2-(2-Phenylethyl) chromone (PEC) and its derivatives are markers of agarwood formation and are also related to agarwood quality. However, the biosynthetic and regulatory mechanisms of PECs still remain mysterious. Several studies suggested that type III polyketide synthases (PKSs) contribute to PEC biosynthesis in Aquilaria sinensis. Furthermore, systematic studies on the evolution of PKSs in A. sinensis have rarely been reported. Herein, we comprehensively analyzed PKS genes from 12 plant genomes and characterized the AsPKSs in detail. A unique branch contained only AsPKS members was identified through evolutionary analysis, including AsPKS01 that was previously indicated to participate in PEC biosynthesis. AsPKS07 and AsPKS08, two tandem-duplicated genes of AsPKS01 and lacking orthologous genes in evolutionary models, were selected for their transient expression in the leaves of Nicotiana benthamiana. Subsequently, PECs were detected in the extracts of N. benthamiana leaves, suggesting that AsPKS07 and AsPKS08 promote PEC biosynthesis. The interaction between the promoters of AsPKS07, AsPKS08 and five basic leucine zippers (bZIPs) from the S subfamily indicated that their transcripts could be regulated by these transcription factors (TFs) and might further contribute to PECs biosynthesis in A. sinensis. Our findings provide valuable insights into the molecular evolution of the PKS gene family in A. sinensis and serve as a foundation for advancing PEC production through the bioengineering of gene clusters. Ultimately, this contribution is expected to shed light on the mechanism underlying agarwood formation.

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