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Physiology, metabolism, antibiotic resistance, and genetic diversity of Harveyi Glade bacteria isolated from coastal mariculture system in China in the last two decades

文献类型： 外文期刊

作者： Kang, Hao 1 ; Yu, Yongxiang 2 ; Liao, Meijie 2 ; Wang, Yingeng 2 ; Yang, Guanpin 1 ; Zhang, Zheng 2 ; Li, Bin 2 ; Rong, Xiaojun 2 ; Wang, Chunyuan 2 ;

作者机构： 1.Ocean Univ China OUC, Coll Marine Life Sci, Qingdao, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Maricultural Organism Dis Control, Qingdao, Peoples R China

3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

关键词： vibrio; harveyi clade; phenotype; multi-locus sequence analysis (MLSA); multi-locus sequence typing (MLST); antibiotic resistance

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：5.247； 五年影响因子：5.72 ）

ISSN：

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： Vibrio bacteria, particularly members of the Harveyi Glade, are the most important pathogens of aquatic organisms that cause significant economic losses in the world. It is difficult to provide specific data on taxa of the Harveyi Glade for biological research and prevention strategies. Therefore, we conducted an extensive phenotypic and antibiotic resistance study, as well as phylogenetic and molecular typing of 192 isolates of the Harveyi Glade collection from 2000 to 2020 with a typical interannual difference from a coastal area in China. The isolates had a significant interspecific genetic and antibiotic resistance diversity. Based on the multilocus sequence analysis (MLSA) of housekeeping genes (gyrB, pyrH, recA, and atpA), 192 Harveyi Glade isolates were rapidly and accurately classified into 10 species. The population of these isolates was composed of 95 sequence types (STs), of which 92 STs were newly identified, indicating a high degree of genetic diversity. ST327 ranked first, accounting for 11.5% of the total number of isolates (22 out of 192), followed by ST215 with 6.25%, while 63 STs included single isolates. At the metabolic level, the physiological and biochemical experiments revealed that all the Harveyi Glade isolates were positive for oxidase and negative for melibiose. The isolates showed a varied tolerance to 11 antibiotics. No isolates were resistant to neomycin. The percentages of sulfadimidine-resistant strains (61 out of 192), sulfadiazine (44 out of 192), sulfamonomethoxine (44 out of 192), sulfamethoxazole (33 out of 192), thiamphenicol (34 out of 192), ciprofloxacin (52 out of 192), and enrofloxacin (31 out of 192) were 31.77%, 22.92%, 22.92%, 17.19%, 17.71%, 27.08%, and 16.15%, respectively. A proportion of 61.8% of the isolates presented a multiple antibiotic resistance index (MARI) lower than 0.1, indicating that the risk of antibiotic resistance transmission of most of the Harveyi Glade is low in mariculture systems in China. These results provide substantial data to support further studies on the identification and genetic and metabolic diversity of Harveyi Glade isolates in mariculture systems in China.

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