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Integration of microbiomics and metabolomics reveals energy metabolism imbalance in crucian carp (Carassius auratus) under saline-alkaline exposure

文献类型： 外文期刊

作者： Han, Lin 1 ; Liu, Wenzhi 1 ; Yuan, Fangying 1 ; Liu, Qianwen 1 ; Cheng, Hongyu 1 ; Jin, Xiaofeng 1 ; Sun, Yanchun 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Areas, Lab Qual & Safety Risk Assessment Aquat Prod Harbi, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Food Sci & Engn, Shanghai 201306, Peoples R China

3.Harbin Univ Sci & Technol, Coll Mat & Chem Engn, Dept Chem Engn & Technol, Harbin 150080, Peoples R China

4.Dalian Ocean Univ, Sch Food Sci & Engn, Dept Food Sci & Engn, Dalian 116023, Peoples R China

关键词： Saline-alkaline stress; Crucian carp; Metabolomics; Microbiology; Intestine

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：4.3； 五年影响因子：4.1 ）

ISSN： 1532-0456

年卷期： 2025 年 291 卷

页码：

收录情况： SCI

摘要： The ecological conditions of freshwater aquaculture are deteriorating by degrees in recent years. Consequently, the comprehensive utilization of saline-alkaline water has garnered increasing societal attention. Here, crucian carp (Carassius auratus) were exposed to 20, 40 mmol/L NaHCO3 for 30 days (T, F group). Metabolomic analyses were conducted using UPLC-QTOF/MS, complemented by biochemical and microbiology profiling to elucidate the damage of the saline environment to the intestinal microbial structure, which in turn interfered with the energy metabolism. It was observed that carbonate alkalinity (CA) exposure not only caused intestine oxidative stress but also changed the levels of several digestive enzymes, including alpha-amylase (AMS), chymotrypsin (CHY), lipase (LPS). Metabolomic analysis identified 22 different metabolites (DEMs) in T group and 77 DEMs in F group. MetaboAnalyst analysis indicated that these metabolites are primarily involved in energy-related pathways, including the citric acid cycle, galactose metabolism, and glycine, serine, and threonine metabolism. Intestinal microbial diversity and community composition were altered under carbonate alkalinity exposure, with increase in Proteobacteria abundance and decline in Firmicutes, abundance alongside enrichment of Sphingomonas. Herein, saline-alkaline stress disrupted the physiological homeostasis of the crucian carp intestine, leading to microbial dysbiosis and energy metabolic imbalance. This study provides a theoretical foundation for understanding the stress response of the crucian carp intestine and the role of the intestinal microbiome in host resilience under adverse environmental conditions.

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