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Evaluation of the transcriptional regulatory efficacy of transcription regulatory sequences of porcine epidemic diarrhea virus

文献类型： 外文期刊

作者： Peng, Qi 1 ; Zhang, Xue 1 ; Fan, Baochao 1 ; Li, Yunchuan 1 ; Zhao, Shuqing 1 ; Guo, Weilu 1 ; He, Wenlong 1 ; Zhao, Yongxiang 1 ; Ni, Yanxiu 1 ; Liu, Maojun 1 ; Fei, Rongmei 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing 210014, Peoples R China

2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Jiangsu Key Lab Zoonoses, Yangzhou 225009, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Dis Diag & Immunol, 1 Weigang, Nanjing 210095, Peoples R China

5.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Nanjing 210094, Peoples R China

6.Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing 210094, Peoples R China

7.Hebei Agr Univ, Coll Vet Med, Baoding 071001, Peoples R China

关键词： PEDV; TRS; Reverse genetic; EGFP

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.246； 五年影响因子：3.565 ）

ISSN： 0378-1135

年卷期： 2022 年 267 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteropathogenic coronavirus causing severe watery diarrhea and high mortality in piglets. In order to investigate the role of the transcription regulatory sequences (TRSs) in regulation of gene expression and replication of PEDV, the enhanced green fluorescent protein (EGFP) gene, under control of different TRSs of PEDV, were inserted between the N gene and 3 & PRIME; UTR of the PEDV genome using a reverse genetic system. The EGFP expression from different chimeric PEDVs was analyzed for each TRS. TRSs of all the structural and accessory protein genes of PEDV positively regulate EGFP expression at different levels, and the TRS of M protein gene produced the highest level of EGFP. Moreover, this is the first study to show that exogenous gene could be inserted between N gene and 3' UTR of PEDV, and the EGFP insertion had no effect on PEDV replication. Taken together, our study enriched the information of PEDV TRSs on gene expression and replication of PEDV.

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