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One-Step Labeling Based on Eu-MOFs to Develop Fluorescence Side-Flow Immunoassay for AFB1 Detection in Corn

文献类型： 外文期刊

作者： Li, Yinjun 1 ; Ding, Hua 1 ; Wang, Ziyu 2 ; Luo, Zewei 2 ; Peng, Xitian 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Hubei Key Lab Nutr Qual & Safety Agro Prod, Wuhan 430064, Hubei, Peoples R China

2.Sichuan Univ, Sch Mech Engn, Res Ctr Analyt Instrumentat, Chengdu 610065, Peoples R China

关键词： lateral flow immunoassay; metal-organic frameworks; aflatoxin B1; rapid detection

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN：

年卷期： 2025 年 15 卷 5 期

页码：

收录情况： SCI

摘要： Lateral flow immunoassay (LFIA) is a promising tool for rapid detection in the field of agricultural product analysis due to its advantages of cost-effectiveness and operational simplicity. In this work, Eu metal-organic frameworks (MOFs) were introduced to LFIA as a rapid detection method characterized by high stability and low interference. Key research objectives included strong fluorescence, ease of labeling, and the utilization of fluorescent probes. Eu-MOFs were synthesized in one step via the hydrothermal method, exhibiting a fluorescence lifetime of 163 mu s and spherical particles with diameters ranging from 250 to 400 nm. These conditions fulfill the characteristics and requirements of LFIA. Eu-MOFs exploit the porous nature of MOFs to mitigate the drawbacks associated with complex crosslinking agents. This enables antibody proteins to be cross-linked merely upon contact, thereby simplifying the detection process. A time-resolved LFIA method was developed utilizing Eu-MOFs for the detection of aflatoxin B1 (AFB1) in corn, achieving a limit of detection (LOD, IC10) of 0.149 ng/mL. The accuracy and reliability of the Eu-MOFs-LFIA method were validated through comparisons with spiked concentrations during spiking and blind sample analyses, with verification conducted using ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS). Furthermore, testing of real samples demonstrated that the Eu-MOFs-LFIA method can effectively facilitate rapid detection of AFB1 in corn.

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