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Transcriptomic responses to air exposure stress in coelomocytes of the sea cucumber, Apostichopus japonicus

文献类型： 外文期刊

作者： Tan, Jie 1 ; Wang, Xuejiang 3 ; Wang, Liang 4 ; Zhou, Xiaoqun 4 ; Liu, Changlin 1 ; Ge, Jianlong 1 ; Bian, Li 1 ; Chen, Siqing 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

3.Wuzhoufeng Agr Sci & Technol Co LTD, 20 Huasheng Rd, Yantai 264000, Shandong, Peoples R China

4.Yantai Marine Econ Res Inst, 2 Kunlunshan Rd, Yantai 264003, Shandong, Peoples R China

关键词： Apostichopus japonicus; Air exposure stress; Coelomocyte; Transcriptional response; RNA-seq

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 2021影响因子：3.306； 五年影响因子：3.251 ）

ISSN： 1744-117X

年卷期： 2022 年 42 卷

页码：

收录情况： SCI

摘要： During rearing in hatcheries and transportation to restocking sites, sea cucumbers are often exposed to air for several hours, which may depress their non-specific immunity and lead to mass mortality. We performed transcriptome analysis of Apostichopus japonicus coelomocytes after air exposure to identify stress-related genes and pathways. After exposure to air for 1 h, individuals were re-submerged in aerated seawater and coelomocytes were collected at 0, 1, 4, and 16 h (B, H1, H4, and H16, respectively). We identified 6148 differentially expressed genes, of which 3216 were upregulated and 2932 were downregulated. Many genes involved in the immune response, antioxidant defense, and apoptosis were highly induced in response to air exposure. Enrichment analysis of Gene Ontology terms showed that the most abundant terms in the biological process category were oxidation-reduction process, protein folding and phosphorylation, and receptor-mediated endocytosis for the comparison of H1 vs. B, H4 vs. H1, and H16 vs. H4, respectively. Kyoto Eecyclopedia of Genes and Genomes enrichment analysis showed that six pathways related to the metabolism of proteins, fats, and carbohydrates were shared among the three comparisons. These results indicated that sea cucumbers regulate the expression of genes related to the antioxidant system and energy metabolism to resist the negative effects of air exposure stress. These findings may be applied to optimize juvenile sea cucumber production, and facilitate molecular marker-assisted selective breeding of an anoxia-resistant strain.

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