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An ultra-high-sensitivity electrochemiluminescence aptasensor for Pb2+ detection based on the synergistic signal-amplification strategy of quencher abscission and G-quadruplex generation

文献类型： 外文期刊

作者： Liu, Xiaohong 1 ; Li, Libo 1 ; Li, Fang 1 ; Zhao, Wanlin 1 ; Luo, Lijun 1 ; Bi, Xiaoya 1 ; Li, Xia 3 ; You, Tianyan 1 ;

作者机构： 1.Jiangsu Univ, Sch Agr Engn, Minist Educ, Key Lab Modern Agr Equipment & Technol, Zhenjiang 212013, Jiangsu, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

3.Liaocheng Univ, Dept Chem, Liaocheng 252059, Shandong, Peoples R China

关键词： Electrochemiluminescence aptasensor; Synergistic signal-amplification strategy; Ru(dcbpy)(3)(2+); Nitrogen-doped carbon quantum dots

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：14.224； 五年影响因子：12.984 ）

ISSN： 0304-3894

年卷期： 2022 年 424 卷

页码：

收录情况： SCI

摘要： Signal amplification provides an effective way to improve detection performance. Herein, an ultrasensitive electrochemiluminescence (ECL) aptasensor for Pb2+ detection was developed based on a dual signal-amplification strategy of the abscission of a quencher and the generation of a G-quadruplex by one-step and simultaneous way. Nitrogen-doped carbon quantum dots linked with complementary DNA (cDNA-NCQDs) at the sensing interface was applied as the quencher of a tris(4,4'-dicarboxylic acid-2,2'-bipyridyl)ruthenium(II) (Ru (dcbpy)(3)(2+))/tripropylamine system to minimize the ECL signal due to the intermolecular hydrogen bond-induced energy-transfer process. Upon the addition of Pb2+, its specific binding with the aptamer triggered the abscission of cDNA-NCQDs, accompanied by the formation of G-quadruplex on the surface of the electrode, both of which amplified the intensity of the light emission. The ECL amplification efficiency induced by the above two mechanisms (78.6%) was valuably greater than that of their sum value (69.3%). This synergistic effect resulted in high detection sensitivity of the ECL aptasensor, which allowed to thereby obtain Pb2+ measurements in the range of 1 fM - 10 nM with an ultra-low detection limit of 0.19 fM. The Pb2+-mediated synergistic signal-amplification ECL strategy can provide a new approach for integrating various amplification strategies.

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