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Destruxin A inhibits scavenger receptor B mediated melanization in Aphis citricola

文献类型： 外文期刊

作者： Han, Pengfei 1 ; Gong, Qitian 1 ; Fan, Jiqiao 3 ; Abbas, Mureed 1 ; Chen, Duo 1 ; Zhang, Jianzhen 1 ;

作者机构： 1.Shanxi Univ, Inst Appl Biol, Taiyuan 030006, Peoples R China

2.Shanxi Univ, Coll Life Sci, Taiyuan, Peoples R China

3.Shanxi Key Lab Integrated Pest Management Agr, Taiyuan, Peoples R China

4.Shanxi Agr Univ, Coll Plant Protect, Taiyuan, Peoples R China

关键词： destruxin A; Aphis citricola; melanization; RNA interference

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.462； 五年影响因子：4.688 ）

ISSN： 1526-498X

年卷期：

页码：

收录情况： SCI

摘要： BACKGROUND Destruxin A (DA) is a mycotoxin secreted by entomogenous fungi, such as Metarhizium anisopliae, which has broad-spectrum insecticidal activity. Insect innate immunity provides resistance against the invasion of entomopathogenic fungi. Previous studies have shown that DA could inhibit the immune response, however, the suppressive mechanism of DA on prophenoloxidase system is still unknown. RESULTS Based on the transcriptome of Aphis citricola, we screened the scavenger receptor class B(AcSR-B)and identified that it significantly responds to DA. Spatio-temporal expression analysis showed that AcSR-B is highly expressed in adult stage and is mainly distributed in the abdominal region. We further revealed that both M. anisopliae and Escherichia coli could suppress the expression of AcSR-B at 24 h, and that the expressed recombinant protein rAcSR-B possessed agglutination activity to M. anisopliae and E. coli. DA could suppress the protein expression of AcSR-B. In addition, RNA interference of AcSR-B caused death of A. citricola in a dose-dependent manner, and RNA interference of AcSR-B increased mortality in A. citricola under the same lethal concentration of DA. The inhibiting effect of AcSR-B silencing was similar with the DA treatment upon phenol oxidase (PO) activity of A. citricola hemolymph. DA could not decrease PO activity further after AcSR-B silencing. CONCLUSION Destruxin A inhibits melanization by suppressing AcSR-B in A. citricola. Our findings are helpful in understanding the underlying molecular mechanism of the DA suppressing immune system, and uncover a potential molecular target for double-stranded RNA (dsRNA) insecticides.

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