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Sgabd-2 plays specific role in immune response against biopesticide Metarhizium anisopliae in Aphis citricola

文献类型： 外文期刊

作者： Fan, Jiqiao 1 ; Jiang, Shirong 2 ; Zhang, Tao 2 ; Gao, Huiyan 2 ; Chang, Babar Hussain 2 ; Qiao, Xiongwu 1 ; Han, Pengfei 2 ;

作者机构： 1.Shanxi Agr Univ, Coll Plant Protect, Shanxi Key Lab Integrated Pest Management Agr, Taiyuan 030031, Peoples R China

2.Shanxi Univ, Inst Appl Biol, Taiyuan 030006, Peoples R China

3.Shanxi Key Lab Nucl Acid Biopesticide, Laser Spect Lab, Taiyuan 030006, Peoples R China

关键词： Aphis citricola; Metarhizium anisopliae; Sgabd-2; Immunity; RNAi; Recombinant fungi

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN： 0048-3575

年卷期： 2024 年 203 卷

页码：

收录情况： SCI

摘要： Metarhizium anisopliae is an effective biopesticide for controlling Aphis citricola, which has developed resistance to many chemical pesticides. However, the powerful immune system of A. citricola has limited the insecticidal efficacy of M. anisopliae. The co-evolution between insects and entomogenous fungi has led to emergence of new antifungal immune genes, which remain incompletely understood. In this study, an important immune gene Sgabd-2 was identified from A. citricola through transcriptome analysis. Sgabd-2 gene showed high expression in the 4th instar nymph and adult stages, and was mainly distributed in the abdominal region of A. citricola. The recombinant protein (rSgabd-2) exhibited no antifungal activity but demonstrated clear agglutination activity towards the conidia of M. anisopliae. RNA interference of Sgabd-2 by dsRNA feeding resulted in decreased phenoloxidase (PO) activity and weakened defense for A. citricola against M. anisopliae. Simultaneous silence of GNBP-1 and Sgabd-2 effectively reduced the immunity of A. citricola against M. anisopliae more than the individual RNAi of GNBP-1 or Sgabd-2. Furthermore, a genetically engineered M. anisopliae expressing doublestranded RNA (dsSgabd-2) targeting Sgabd-2 in A. citricola successfully suppressed the expression of Sgabd-2 and demonstrated increased virulence against A. citricola. Our findings elucidated Sgabd-2 as a critical new antifungal immune gene and proposed a genetic engineering strategy to enhance the insecticidal virulence of entomogenous fungi through RNAi-mediated inhibition of pest immune genes.

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