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Single-cell RNA-seq offer new insights into the cell fate decision of the primordial germ cells

文献类型： 外文期刊

作者： Zou, Xian 1 ; He, Yanhua 1 ; Zhao, Zhifeng 1 ; Li, Jianbo 4 ; Qu, Hao 1 ; Liu, Zijing 1 ; Chen, Peng 1 ; Ji, Jian 1 ; Zhao, Haoyi 1 ; Shu, Dingming 1 ; Luo, Chenglong 1 ;

作者机构： 1.Guangdong Acad Agr Sci, State Key Lab Swine & Poultry Breeding Ind, Guangzhou 510640, Peoples R China

2.Guangdong Acad Agr Sci, Guangdong Key Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China

3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China

4.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Peoples R China

关键词： PGCs; Single-cell RNA-seq; Cell cycle; Small molecule; Proliferation; High efficiency

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 293 卷

页码：

收录情况： SCI

摘要： The faithful production of primordial germ cells (PGCs) in vitro opens a wide range of novel applications in reproductive biology and medicine. However, the reproducibility of PGCs culture conditions across different laboratories or breeds remains a challenge. Therefore, it is necessary to research the molecular dynamics that lead to the gradual establishment of cultured PGCs lines network. Here, the results of single-cell RNA-seq indicated that the cell cycle drove cellular heterogeneity. The active populations engaged in PGC self-renewal and the characteristics of the aging cell fate have been identified. The active self-renewal populations presented a rising expression of DNA repair genes, couple with a high proportion of cells in G1/S phase and a low frequency of cells in G2 phase. Notably, Hippo, FoxO, AMPK and MAPK pathways are active within these populations. The combination of six activator or inhibitors, targeting these active pathways, resulted in a significantly higher proliferation rate of PGCs and an increased number of cells entering the G1 and S phases. Importantly, they greatly reduced the establishment time to a minimum of 26 days and increased the efficiency of male PGC line establishment to 59 % in FS medium. Our results provided several new insights into the PGCs.

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