Gravity-driven and rotation-controlled microfluidic chip for point-of-care nucleic acid detection in the fully closed environment
文献类型： 外文期刊
作者： Chen, Yanju 1 ; Zhu, Yuanyuan 1 ; Wang, Xiaofu 2 ; Peng, Cheng 2 ; Wang, Rui 3 ; Wang, Tingzhang 4 ; Che, Yang 4 ; Wu, Jian 1 ; Xu, Junfeng 2 ;
作者机构： 1.Zhejiang Univ, Coll Biosyst Engn & Food Sci, 866 Yuhangtang Rd, Hangzhou 310058, Peoples R China
2.Zhejiang Acad Agr Sci, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China
3.Fudan Univ, Human Phenome Inst, State Key Lab Genet Engn, Shanghai 200438, Peoples R China
4.Zhejiang Inst Microbiol, Key Lab Microbiol Technol & Bioinformat, Hangzhou 310012, Peoples R China
5.Zhejiang Univ, ZJU Hangzhou Global Sci & Technol Innovat Ctr, Hangzhou 311215, Peoples R China
关键词： Microfluidic chip; Closed environment; Gravity; Rotation; Point-of-care; Self-testing
期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）
ISSN： 0039-9140
年卷期： 2024 年 267 卷
页码：
收录情况： SCI
摘要： Point-of-care nucleic acid detection is essential for diagnosis and food safety, especially in resource-limited areas. This study reports a g ravity-driven and r otation-controlled (GR) chip-coupled lateral flow-based assay (LFA) for point-of-care nucleic acid detection. The sample solution is added to the inlet of the GR chip and flows into the loop-medicated isothermal amplification (LAMP) chamber by the action of gravity. After the LAMP reaction, the GR chip is manually rotated to allow the amplified solution to flow into the LFA chamber for result readout. The GR chip integrates the LAMP reaction and LFA in a fully closed environment, avoiding the aerosol contamination problem. The GR chip's simple and ingenious structure simplifies the fabrication and reduces its cost, making it possible for point-of-care and even home self-testing.
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