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Genomic and Comparative Transcriptomic Analyses Reveal Key Genes Associated with the Biosynthesis Regulation of Okaramine B in Penicillium daleae NBP-49626

文献类型： 外文期刊

作者： Wang, Yueying 1 ; Chen, Ling 1 ; Fang, Wei 1 ; Zeng, Zhen 1 ; Wu, Zhaoyuan 1 ; Liu, Fang 1 ; Liu, Xiaoyan 1 ; Gong, Yan 1 ; Zhu, Lei 1 ; Wang, Kaimei 1 ;

作者机构： 1.Hubei Acad Agr Sci, Natl Biopesticide Engn Technol Res Ctr, Hubei Biopesticide Engn Res Ctr, Key Lab Microbial Pesticides,Minist Agr & Rural Af, Wuhan 430064, Peoples R China

关键词： Okaramine B; yield improvement; Penicillium daleae; complete genome; transcriptome; novel regulators; tryptophan metabolism

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 2022影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 4 期

页码：

收录情况： SCI

摘要： Restricted production of fungal secondary metabolites hinders the ability to conduct comprehensive research and development of novel biopesticides. Okaramine B from Penicillium demonstrates remarkable insecticidal efficacy; however, its biosynthetic yield is low, and its regulatory mechanism remains unknown. The present study found that the yield difference was influenced by fermentation modes in okaramine-producing strains and performed genomic and comparative transcriptome analysis of P. daleae strain NBP-49626, which exhibits significant features. The NBP-49626 genome is 37.4 Mb, and it encodes 10,131 protein-encoding genes. Up to 5097 differentially expressed genes (DEGs) were identified during the submerged and semi-solid fermentation processes. The oka gene cluster, lacking regulatory and transport genes, displayed distinct transcriptional patterns in response to the fermentation modes and yield of Okaramine B. Although transcription trends of most known global regulatory genes are inconsistent with those of oka, this study identified five potential regulatory genes, including two novel Zn(II)2Cys6 transcription factors, Reg2 and Reg19. A significant correlation was also observed between tryptophan metabolism and Okaramine B yields. In addition, several transporter genes were identified as DEGs. These results were confirmed using real-time quantitative PCR. This study provides comprehensive information regarding the regulatory mechanism of Okaramine B biosynthesis in Penicillium and is critical to the further yield improvement for the development of insecticides.

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