文献类型： 外文期刊
作者： Li, Tong 1 ; Zhang, Xue-Meng 1 ; Gao, Jia-Lu 1 ; Wang, Ling 2 ; Si, Liang 1 ; Shu, Yong-Jun 3 ; Guo, Chang-Hong 3 ; Lai, Yong-Cai 2 ; Bi, Ying-Dong 2 ; Guo, Dong-Lin 1 ;
作者机构： 1.Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150025, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Crops Tillage & Cultivat, Harbin 150086, Peoples R China
3.Key Lab Mol Cytogenet & Genet Breeding Heilongjian, Harbin 150025, Peoples R China
关键词： soybean; GmVIT1; transporter; iron; manganese
期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN：
年卷期： 2023 年 13 卷 2 期
页码：
收录情况： SCI
摘要： Iron (Fe) and (Mn) are essential for the plant but are toxic when in excess. Vacuolar iron transporters (VITs) are involved in plant metal storage and detoxication. In this study, we screened two soybean cultivars (HN51 and SN37) with different responses to iron stress. From HN51 and SN37, we identified a new gene GmVIT1, for which expression is closely related to iron stress response by transcriptomic and quantitative analysis. We obtained GmVIT1 and GmVIT1 promoter from the iron deficiency-tolerant soybean variety Heinong51. Sequence analysis showed that GmVIT1 contained a conserved 170-residue VIT domain and localized at the tonoplast. Moreover, GmVIT1 is expressed in soybean leaves, stems, and roots. The expression of GmVIT1 was significantly induced by excessive Fe/Mn in leaves and stems. GUS assay showed that excess Fe/Mn enhanced GmVIT1 promoter activity. Furthermore, overexpression of GmVIT1 in Arabidopsis seedlings showed reduced phytotoxic effects induced by excess Fe/Mn stress, including yellowing in leaves, decreased chlorophyll content, and accumulated MDA. GmVIT1 overexpression in Arabidopsis showed relatively higher soluble sugar content and SOD, POD, and CAT activity. In addition, the ferric reductase activity in GmVIT1 overexpression in Arabidopsis decreased under excess Fe, while it increased under excess Mn. By integrating all these results, we found that GmVIT1 plays a vital role in plant response to excess Fe/Mn. The results showed that GmVIT1 was worthy of metal homeostasis mechanism research in plants and could be applied in the metal toxic-tolerance improvement in crops.
- 相关文献
作者其他论文 更多>>
-
Alfalfa MsbHLH115 confers tolerance to cadmium stress through activating the iron deficiency response in Arabidopsis thaliana
作者：Zhang, Miao;Gao, Jing-Yun;Dong, Shi-Chen;Chang, Meng-Han;Zhu, Jing-Xuan;Guo, Dong-Lin;Guo, Chang-Hong;Bi, Ying-Dong
关键词：cadmium; iron nutrient; MsbHLH115; transcription factors; alfalfa
-
An inducible CRISPR activation tool for accelerating plant regeneration
作者：Zhang, Cuimei;Xu, Yujun;Zhou, Yangyan;Liu, Qikun;Tang, Yajun;Yuan, Haidi;Zhou, Junhui;Tang, Shanjie;Chen, Lei;Li, Tong;Zhang, Shuaibin;Deng, Xian;Cao, Xiaofeng;Song, Xianwei;Wang, Jianli;Wen, Hongyu;Jiang, Wenbo;Pang, Yongzhen;Deng, Xian;Song, Xianwei
关键词：inducible CRISPR activation; morphogenic gene; genetic transformation; plant regeneration
-
MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
作者：Li, Run-Tian;Yang, Yun-Jiao;Liu, Wen-Jun;Liang, Wen-Wei;Zhang, Miao;Dong, Shi-Chen;Shu, Yong-Jun;Guo, Dong-Lin;Guo, Chang-Hong;Liang, Wen-Wei;Bi, Ying-Dong
关键词：MsNRAMP2; iron excess; MsMYB; alfalfa; promoter; tobacco
-
Land use intensification alters the relative contributions of plant functional diversity and soil properties on grassland productivity
作者：Zhang, Minna;Li, Guangyin;Wang, Yue;Wang, Ling;Pan, Duofeng;Sun, Jinyan
关键词：Plant diversity; Productivity; Land use intensification; Functional traits; Soil properties
-
Global analysis of seed transcriptomes reveals a novel PLATZ regulator for seed size and weight control in soybean
作者：Hu, Yang;Liu, Yue;Lu, Long;Tao, Jian-Jun;Cheng, Tong;Jin, Meng;Wang, Zhou-Ya;Wei, Jun-Jie;Jiang, Zhi-Hao;Yin, Cui-Cui;Wei, Wei;Zhang, Wan-Ke;Chen, Shou-Yi;Zhang, Jin-Song;Hu, Yang;Liu, Yue;Cheng, Tong;Jin, Meng;Wang, Zhou-Ya;Wei, Jun-Jie;Jiang, Zhi-Hao;Zhang, Jin-Song;Sun, Wan-Cai;Liu, Cheng-Lan;Gao, Feng;Chen, Shou-Yi;Zhang, Yong;Li, Wei;Bi, Ying-Dong;Lai, Yong-Cai;Zhou, Bin;Yu, De-Yue
关键词：cell cycle; gibberellin; PLATZ; seed size; seed weight; soybean
-
Efficient CRISPR/Cas9-mediated genome editing in sheepgrass (Leymus chinensis)
作者：Lin, Zhelong;Chen, Lei;Tang, Shanjie;Zhao, Mengjie;Li, Tong;You, Changqing;Zhao, Qinghua;Song, Xianwei;Zhang, Shuaibin;Cao, Xiaofeng;Lin, Zhelong;Chen, Lei;Tang, Shanjie;Zhao, Mengjie;Li, Tong;You, Changqing;Zhao, Qinghua;Song, Xianwei;Zhang, Shuaibin;Cao, Xiaofeng;You, Jia;Zhang, Dongmei;Wang, Jianli;Shen, Zhongbao;Li, Boshu
关键词：Agrobacterium; genetic transformation; genome editing; LcTB1; sheepgrass
-
Telomere-to-telomere genome of the allotetraploid legume Sesbania cannabina reveals transposon-driven subgenome divergence and mechanisms of alkaline stress tolerance
作者：Luo, Haofei;Wang, Xiaofei;You, Changqing;Wu, Xuedan;Li, Tong;He, Kaixuan;Ye, Qingtong;Zhao, Qinghua;Deng, Xian;Cao, Xiaofeng;Song, Xianwei;Huang, Gai;Luo, Haofei;Wang, Xiaofei;You, Changqing;Wu, Xuedan;Li, Tong;He, Kaixuan;Ye, Qingtong;Zhao, Qinghua;Deng, Xian;Cao, Xiaofeng;Song, Xianwei;Huang, Gai;Wang, Xiaofei;Lv, Zhiyao;He, Kaixuan;Jia, Yajun;You, Changqing;Ye, Qingtong;Cao, Xiaofeng;Pan, Duofeng;Zhang, Dongmei;Shen, Zhongbao;Zhang, Xiaodong;Zhang, Xiaodong;Zhang, Xiaodong;Liu, Guodao
关键词：Sesbania cannabina; alkaline stress; telomere-to-telomere genome; legume; green manure; phosphate transporter; allotetraploid
