您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Selection of a Single Chain Variable Fragment Antibody against Ivermectin from a Phage Displayed Library

文献类型： 外文期刊

作者： Wen, Shuang 1 ; Zhang, Xiao 1 ; Liu, Yuan 1 ; Zhang, Qingqing 1 ; Liu, Xianjin 1 ; Liang, Jiansheng 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, Key Lab Food Safety Monitoring & Management, Minist Agr, Nanjing 210014, Peoples R China

2.Yangzhou Univ, Key Lab Crop Genet & Physiol Jiangsu Prov, Coll Biosci & Biotechnol, Yangzhou 225009, Jiangsu, Peoples R China

关键词： IVM;scFv;detection;phage displayed library

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Single chain variable fragment antibodies (scFvs) have considerable potential in immunological detection of pesticides, veterinary and other residues. In this study, a large human synthetic phage displayed library was used to select scFvs against ivermectin (IVM) by subtractive panning. After four rounds of panning, seven monoclonal phage particles capable of binding with the IVM were obtained. One of the identified novel anti-IVM scFv, hslVM8, was expressed in Escherichia coli HB2151 and purified with Ni metal ion affinity chromatography. Sodium dodecyl sulfate polyacry-lamine gel electrophoresis (SDS-PAGE) indicated that the relative molecular mass of hslVM8 was estimated at 28 kDa. The purified hslVM8 was used to develop a competitive indirect enzyme-linked immunosorbent assay (ci-ELISA) for IVM. The linear range of detection for standards in this ci-ELISA was approximately 0.1-5 μg/mL and 50% inhibition of control (IC_(50)) was 4.11 μg/mL for IVM. These results showed promising applications of hslVM8 for detecting IVM in agricultural and environmental matrices.

  • 相关文献

[1]Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis (Bt) Cry1B toxin. Zhang, Xiao,Liu, Yuan,Zhang, Cunzheng,Wang, Yun,Liu, Xianjin,Zhang, Xiao,Wang, Yun,Xu, Chongxin. 2012

[2]Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries. Wang, Yun,Zhang, Xiao,Liu, Xianjin,Wang, Yun,Zhang, Xiao,Zhang, Cunzheng,Liu, Yuan,Liu, Xianjin.

[3]单链抗体对Cry1A类毒素的检测方法建立及识别差异初步研究. 曲婷婷,张霄,董飒,刘贝贝,李盼,王耘,张存政,刘贤金. 2019

[4]Construction of scFv phage display library with hapten-specific repertories and characterization of anti-ivermectin fragment isolated from the library. Zhang, Xiao,Zhang, Cunzheng,Liu, Yuan,Yu, Xiangyang,Liu, Xianjin. 2010

[5]Established a new double antibodies Sandwich enzyme-linked immunosorbent assay for detecting Bacillus thuringiensis (Bt) Cry1Ab toxin based single-chain variable fragments from a naive mouse phage displayed library. Zhang, Xiao,Xu, Chongxin,Zhang, Cunzheng,Liu, Yuan,Xie, Yajing,Liu, Xianjin. 2014

[6]IMMOBILIZATION OF DYE-DECOLORIZING PEROXIDASE ON MAGNETIC NANOPARTICLES: A DUAL-FUNCTIONAL BIOCATALYST FOR MYCOTOXINS DEGRADATION AND HYDROGEN PEROXIDE DETECTION. Yu Xia,Yangyu Qiu,Yang You,Lili Zhang,Zhouping Wang. 2023

[7]Aptamer-Based Fluorescence Assay for Hg2+ Determination. Lei Zhao-Jing,Hu Qiu-Hui,Lei Zhao-Jing,Zhang Cun-Zheng,Liu Yuan,Zhang Qiang,Liu Xian-Jin. 2012

[8]Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. Guo, Yingpeng,Li, Wei,Sun, Haiyan,Wang, Ning,Chen, Huaigu,Guo, Yingpeng,Yu, Hanshou. 2012

[9]Fluorescence polarization assay for the simultaneous determination of bisphenol A, bisphenol F and their diglycidyl ethers in canned tuna. Guan, Tianzhu,Li, Tiezhu,Li, Zhuolin,Wang, Yongzhi,Zhang, Jie,Wang, Yongjun,Zhang, Tiehua,Yu, Hansong,Ruan, Ping. 2017

[10]Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Xiao, Shao-bo. 2012

[11]Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Mycoplasma hyorhinis. Du, Gai-Mei,Liu, Mao-Jun,Wu, Yu-Zi,Xiong, Qi-Yan,Bai, Fang-Fang,Feng, Zhi-Xin,Shao, Guo-Qing,Du, Gai-Mei. 2013

[12]Development of a loop-mediated isothermal amplification assay for the detection of porcine hokovirus. Li, Bin,Sun, Bing,Du, Lu-ping,Mao, Ai-hua,Wen, Li-bin,Ni, Yan-xiu,Zhang, Xue-han,He, Kong-wang. 2013

[13]Reverse transcription loop-mediated isothermal amplification of RNA for sensitive and rapid detection of southern rice black-streaked dwarf virus. Zhou, Tong,Du, Linlin,Fan, Yongjian,Zhou, Yijun,Zhou, Tong,Du, Linlin,Zhou, Yijun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台