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Effect of high alkalinity on shrimp gills: Histopathological alternations and cell specific responses

文献类型： 外文期刊

作者： Ge, Qianqian 1 ; Wang, Jiajia 1 ; Li, Jitao 1 ; Li, Jian 1 ;

作者机构： 1.Laoshan Lab, Qingdao, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Qingdao Marine Sci & Technol Ctr, Qingdao, Peoples R China

关键词： Alkalinity stress; Gills; Exopalaemon carinicauda; ScRNA-seq; Pillar cell; Nephrocyte

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 256 卷

页码：

收录情况： SCI

摘要： High alkalinity stress was considered as a major risk factor for aquatic animals surviving in saline-alkaline water. However, few information exists on the effects of alkalinity stress in crustacean species. As the dominant role of gills in osmotic and ionic regulation, the present study firstly evaluated the effect of alkalinity stress in Exopa-laemon carinicauda to determine changes in gill microstructure, and then explore the heterogeneity response of gill cells in alkalinity adaptation by single-cell RNA sequencing (scRNA-seq). Hemolymph osmolality and pH were increased remarkably, and gills showed pillar cells with more symmetrical arrangement and longer lateral flanges and nephrocytes with larger vacuoles in high alkalinity. ScRNA-seq results showed that alkalinity stress reduced the proportion of pillar cells and increased the proportion of nephrocytes significantly. The differentially expressed genes (DEGs) related to ion transport, especially acid-base regulation, such as V(H+)-ATPases and carbonic anhydrases, were down-regulated in pillar cells and up-regulated in nephrocytes. Furthermore, pseu-dotime analysis showed that some nephrocytes transformed to perform ion transport function in alkalinity adaption. Notedly, the positive signals of carbonic anhydrase were obviously observed in the nephrocytes after alkalinity stress. These results indicated that the alkalinity stress inhibited the ion transport function of pillar cells, but induced the active role of nephrocytes in alkalinity adaptation. Collectively, our results provided the new insight into the cellular and molecular mechanism behind the adverse effects of saline-alkaline water and the saline-alkaline adaption mechanism in crustaceans.

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