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Analysis of microRNA expression profiles during the differentiation of chicken embryonic stem cells into male germ cells

文献类型： 外文期刊

作者： Li, Xin 1 ; Jin, Haiguo 1 ; Lv, Yang 2 ; Liu, Chen 3 ; Luo, Xiaotong 4 ; Liu, Jianqiang 1 ; Zhang, Qi 1 ; Yu, Yongsheng 1 ; Zhao, Zhongli 3 ;

作者机构： 1.Jilin Acad Agr Sci, Inst Anim Biotechnol, Gong Zhu Ling, Jilin, Peoples R China

2.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China

3.Jilin Acad Agr Sci, Inst Anim Husb & Vet Med, Gong Zhu Ling, Jilin, Peoples R China

4.Yanbian Univ, Agr Coll, Yanji, Peoples R China

关键词： Chicken embryonic stem cells; male germ cells; microRNA; dual-luciferase reporter vector; STRN3

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.141； 五年影响因子：1.92 ）

ISSN： 1049-5398

年卷期：

页码：

收录情况： SCI

摘要： The differentiation of embryonic stem cells (ESCs) into germ cells in vitro could have very promising applications for infertility treatment and could provide an excellent model for uncovering the molecular mechanisms of germline generation. This study aimed to investigate the differentially expressed miRNAs (DEMs) during the differentiation of chicken ESCs (cESCs) into male germ cells and to establish a profile of the DEMs. Cells before and after induction were subjected to miRNA sequencing (miRNA-seq). A total of 113 DEMs were obtained, including 61 upregulated and 52 downregulated DEMs. GO and KEGG enrichment analyses showed that the target genes were enriched mainly in the MAPK signaling pathway, HTLV infection signaling pathway, cell adhesion molecule (CAM)-related pathways, viral myocarditis, Wnt signaling pathway, ABC transporters, TGF-beta signaling pathways, Notch signaling pathways and insulin signaling pathway. The target genes of the miRNAs were related to cell binding, cell parts and biological regulatory processes. Six DEMs, let-7k-5p, miR-132c-5p, miR-193a-5p, miR-202-5p, miR-383-5p and miR-6553-3p, were assessed by qRT-PCR, and the results were consistent with the results of miRNA-seq. Based on qRT-PCR and western blot verification, miR-383-5p and its putative target gene STRN3 were selected to construct an STRN3 3'-UTR dual-luciferase gene reporter vector and its mutant vector. The double luciferase reporter activity of the cotransfected STRN3-WT + miR-383-5p mimics group was significantly lower (by approximately 46%) than that of the other five groups (p < 0.01). There was no significant difference in luciferase activity among the other 5 groups. This study establishes a DEM profile during the process of cESC differentiation into male germ cells; illustrates the mechanisms by which miRNAs regulate target genes; provides a theoretical basis for further research on the mechanisms of the formation and regulation of male germ cells; and provides an important strategy for gene editing, animal genetic resource protection and transgenic animal production.

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