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Proteome Analysis in PAM Cells Reveals That African Swine Fever Virus Can Regulate the Level of Intracellular Polyamines to Facilitate Its Own Replication through ARG1

文献类型： 外文期刊

作者： Ai, Qiangyun 1 ; Lin, Xiwei 1 ; Xie, Hangao 1 ; Li, Bin 5 ; Liao, Ming 1 ; Fan, Huiying 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

2.South China Agr Univ, Res Ctr African Swine Fever Prevent & Control, Guangzhou 510642, Peoples R China

3.Key Lab Zoonosis Prevent & Control Guangdong Prov, Guangzhou 510642, Peoples R China

4.Minist Agr, Key Lab Anim Vaccine Dev, Guangzhou 510642, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing 210014, Peoples R China

关键词： proteomics; ASFV; ARG1; polyamine

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2021 年 13 卷 7 期

页码：

收录情况： SCI

摘要： In 2018, African swine fever broke out in China, and the death rate after infection was close to 100%. There is no effective and safe vaccine in the world. In order to better characterize and understand the virus-host-cell interaction, quantitative proteomics was performed on porcine alveolar macrophages (PAM) infected with ASFV through tandem mass spectrometry (TMT) technology, high-performance liquid chromatography (HPLC), and mass spectrometry (MS). The proteome difference between the simulated group and the ASFV-infected group was found at 24 h. A total of 4218 proteins were identified, including 306 up-regulated differentially expressed proteins and 238 down-regulated differentially expressed proteins. Western blot analysis confirmed changes in the expression level of the selected protein. Pathway analysis is used to reveal the regulation of protein and interaction pathways after ASFV infection. Functional network and pathway analysis can provide an insight into the complexity and dynamics of virus-host cell interactions. Further study combined with proteomics data found that ARG1 has a very important effect on ASFV replication. It should be noted that the host metabolic pathway of ARG1-polyamine is important for virus replication, revealing that the virus may facilitate its own replication by regulating the level of small molecules in the host cell.

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