Isolation and characterization of new microsatellite markers from the Japanese scallop (Patinopecten yessoensis)
文献类型: 外文期刊
作者: Chen, Meng 1 ; Chang, Ya-Qing 1 ; Ding, Jun 1 ; Sun, Xiao-Wen 2 ;
作者机构: 1.DaLian Fisheries Univ, Key Lab Mariculture, Dalian 116023, Liaoning, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Harbin 150070, Peoples R China
关键词: Japanese scallop;Microsatellite;Population structure;Twice hybridizations
期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Twenty polymorphic microsatellite loci were isolated from the Japanese scallop (Patinopecten yessoensis) using a method of hybridizing twice. The genetic diversity was analyzed by these microsatellite markers in field specimens from Zhangzidao Island in Liaoning Province, China. Observed heterozygosity (H_o), expected heterozygosity (H_e), number of effective alleles (N_e), and polymorphism information contents (PIC) were calculated. The heterozygote deficiency or excess was detected by the fixation index (Fis). The Hardy-Weinberg equilibrium was tested by possibilities (P_(HWE)) using chi-square test. Results indicate that the average of allele numbers was 6. 0. The mean values of the parameters H_o, H_e, N_e, and PIC were 0. 7921, 0. 7076, 4. 4347, and 0. 6684, respectively. Only 4 of 20 microsatellite markers showed heterozygote deficiency including loci FJ262370, FJ262381, FJ262384, and FJ262400. The P_(HWE) value indicates the wild population deviated somewhat from the Hardy-Weinberg equilibrium. These newly isolated markers increase the available molecular resources of the Japanese scallop.
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