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A monoterpene synthase gene cluster of tea plant (Camellia sinensis) potentially involved in constitutive and herbivore-induced terpene formation

文献类型： 外文期刊

作者： Qiao, Dahe 1 ; Tang, Mengsha 1 ; Jin, Ling 1 ; Mi, Xiaozeng 1 ; Chen, Hongrong 1 ; Zhu, Junyan 1 ; Liu, Shengrui 1 ; Wei, Chaoling 1 ;

作者机构： 1.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Anhui Prov Lab Tea Plant Biol & Utilizat, Key Lab Tea Biol & Tea Proc,Minist Agr, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

2.Guizhou Acad Agr Sci, Tea Res Inst, 1 Jinnong Rd, Guiyang 550006, Guizhou, Peoples R China

关键词： Biosynthetic gene clusters; Terpene synthase; Camellia sinensis; Geraniol; Linalool; Herbivore-induced plant volatiles

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）

ISSN： 0981-9428

年卷期： 2022 年 184 卷

页码：

收录情况： SCI

摘要： Monoterpenes and sesquiterpenes are the most abundant volatiles in tea plants and have dual functions in aroma quality formation and defense responses in tea plants. Terpene synthases (TPS) are the key enzymes for the synthesis of terpenes in plants; however, the functions of most of them in tea plants are still unknown. In this study, six putative terpene biosynthesis gene clusters were identified from the tea plant genome. Then we cloned three new TPS-b subfamily genes, CsTPS08, CsTPS10 and CsTPS58. In vitro enzyme assays showed that CsTPS08 and CsTPS58 are two multiple-product terpene synthases, with the former synthesizing linalool as the main product, and beta-myrcene, alpha-phellandrene, alpha-terpinolene, D-limonene, cis-beta-ocimene, trans-beta-ocimene and (4E,6Z)allo-ocimene as minor products are also detected, while the latter catalyzing the formation of alpha-pinene and Dlimonene using GPP as the substrate. No product of CsTPS10 was detected in the prokaryotic expression system, but geraniol production was detected when transiently expressed in tobacco leaves. CsTPS08 and CsTPS10 are two functional members of a monoterpene synthase gene cluster, which were significantly induced during both Ectropis oblique feeding and fresh leaf spreading treatments, suggesting that they have dual functions involved in tea plant pest defense and tea aroma quality regulation. In addition, the differences in their expression levels in different tea plant cultivars provide a possibility for the subsequent screening of tea plant resources with a specific aroma flavor. Our results deepen the understanding of terpenoid synthesis in tea plants.

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