A Novel Quantum Dot-based Fluoroimmunoassay Method For Detection Of Enrofloxacin Residue In Chicken Muscle Tissue
文献类型： 外文期刊
作者： Chen, Junxia 1 ; Xu, Fei 1 ; Jiang, Haiyang 1 ; Hou, Yali 2 ; Rao, Qinxiong 3 ; Guo, Pengju 4 ; Ding, Shuangyang 1 ;
作者机构： 1.China Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residue, Beijing 100094, Peoples R China
2.Chongqing Inst Vet Drug & Feed Inspect, Chongqing 401147, Peoples R China
3.Shanghai Acad Agr Sci, Inst Agr Food Standards & Testing, Shanghai 201100, Peoples R China
4.Australian Anim Hlth Lab, CSIRO, Livestock Ind, Geelong, Vic, Australia
关键词： enrofloxacin;quantum dots;chicken muscle;fluoroimmunoassay
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
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收录情况： SCI
摘要： A rapid indirect competitive fluorescence-linked immunosorbent assay (cFLlSA) based on quantum dots (QDs) as the fluorescent marker has been developed for the detection of Enrofloxacin (ENR) in chicken muscle tissue. The end-point fluorescent detection system was carried out using QDs conjugated with goat anti-mouse secondary antibody. The cFLISA method allowed for ENR determination in a liner working range of 1-100 ng mL~(-1) with the 50% inhibition value (IC_(50)) of 8.3 ng mL~(-1) and the limit of detection (LOD) of 2.5 ng mL~(-1). The recoveries for chicken muscle samples spiked with ENR at levels of 50-200 μg kg~(-1) ranged from 81% to 94% with coefficients of variation (CV) of 10-13%. In real chicken tissue sample analysis, the results of cFLISA were similar to those obtained from an indirect competitive enzyme-linked immunosorbent assay (cEUSA) to a high performance liquid chrornatography method (HPLC), which indicated that cFLISA is suitable as screening method for the monitoring of veterinary drug residues.
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