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ThSCSP_12: Novel Effector in Tilletia horrida That Induces Cell Death and Defense Responses in Non-Host Plants

文献类型： 外文期刊

作者： Shu, Xinyue 1 ; Yin, Desuo 2 ; Liang, Juan 1 ; Xu, Deze 2 ; Jiang, Yuqi 1 ; Xiang, Ting 1 ; Wang, Yuxuan 1 ; Jiao, Chunhai 2 ; Li, Ping 3 ; Zheng, Aiping 1 ; Wang, Aijun 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Agron, Chengdu 611130, Peoples R China

2.Hubei Acad Agr Sci, Food Crop Res Inst, Wuhan 430064, Peoples R China

3.Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China

关键词： rice; Tilletia horrida; effector; defense response; interactions

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 23 期

页码：

收录情况： SCI

摘要： The basidiomycete fungus Tilletia horrida causes rice kernel smut (RKS), a crucial disease afflicting hybrid-rice-growing areas worldwide, which results in significant economic losses. However, few studies have investigated the pathogenic mechanisms and functions of effectors in T. horrida. In this study, we found that the candidate effector ThSCSP_12 caused cell necrosis in the leaves of Nicotiana benthamiana. The predicted signal peptide (SP) of this protein has a secreting function, which is required for ThSCSP_12 to induce cell death. The 1- 189 amino acid (aa) sequences of ThSCSP_12 are sufficient to confer it the ability to trigger cell death in N. benthamiana. The expression of ThSCSP_12 was induced and up-regulated during T. horrida infection. In addition, we also found that ThSCSP_12 localized in both the cytoplasm and nucleus of plant cells and that nuclear localization of this protein is required to induce cell death. Furthermore, the ability of ThSCSP_12 to trigger cell death in N. benthamiana depends on the (RAR1) protein required for Mla12 resistance but not on the suppressor of the G2 allele of Skp1 (SGT1), heat shock protein 90 (HSP90), or somatic embryogenesis receptor-like kinase (SERK3). Crucially, however, ThSCSP_12 induced a defense response in N. benthamiana leaves; yet, the expression of multiple defense-related genes was suppressed in response to heterologous expression in host plants. To sum up, these results strongly suggest that ThSCSP_12 operates as an effector in T. horrida-host interactions.

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