文献类型： 外文期刊
作者： Chen, Pingli 1 ; Lou, Guangming 1 ; Wang, Yufu 1 ; Chen, Junxiao 7 ; Chen, Wengfeng 3 ; Fan, Zhilan 3 ; Liu, Qing 3 ; Sun, Bingrui 3 ; Mao, Xingxue 3 ; Yu, Hang 3 ; Jiang, Liqun 3 ; Zhang, Jing 3 ; Lv, Shuwei 3 ; Xing, Junlian 3 ; Pan, Dajian 3 ; Li, Chen 3 ; He, Yuqing 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China
3.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou 510640, Peoples R China
4.Guangdong Key Lab New Technol Rice Breeding, Guangzhou 510640, Peoples R China
5.Guangdong Rice Engn Lab, Guangzhou 510640, Peoples R China
6.Minist Agr & Rural Affairs, Coconstruct Minist & Prov, Key Lab Genet & Breeding High Qual Rice Southern C, Guangzhou 510640, Peoples R China
7.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Wuhan 430070, Peoples R China
关键词： Oryza sativa; Grain protein content; GWAS; Flo5; Grain quality; CRISPR; Cas9
期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）
ISSN： 1380-3743
年卷期： 2023 年 43 卷 1 期
页码：
收录情况： SCI
摘要： The grain protein content (GPC) of rice is an important factor that determines its nutritional, cooking, and eating qualities. To date, although a number of genes affecting GPC have been identified in rice, most of them have been cloned using mutants, and only a few genes have been cloned in the natural population. In this study, 135 significant loci were detected in a genome-wide association study (GWAS), many of which could be repeatedly detected across different years and populations. Four minor quantitative trait loci affecting rice GPC at four significant association loci, qPC2.1, qPC7.1, qPC7.2, and qPC1.1, were further identified and validated in near-isogenic line F-2 populations (NIL-F-2), explaining 9.82, 43.4, 29.2, and 13.6% of the phenotypic variation, respectively. The role of the associated flo5 was evaluated with knockdown mutants, which exhibited both increased grain chalkiness rate and GPC. Three candidate genes in a significant association locus region were analyzed using haplotype and expression profiles. The findings of this study will help elucidate the genetic regulatory network of protein synthesis and accumulation in rice through cloning of GPC genes and provide new insights on dominant alleles for marker-assisted selection in the genetic improvement of rice grain quality.
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