Exosomal circ_CCDC7/gga-miR-6568-3p/Pax7 axis accelerates the differentiation of chicken embryonic stem cells infected with subgroup J avian leukosis virus
文献类型： 外文期刊
作者： Zeng, Xiaona 1 ; Wang, Ruonan 1 ; Tang, Shengqiu 3 ; Dong, Xiaoying 3 ; Liao, Liqin 2 ; Chen, Sheng 1 ; Kong, Jie 2 ; Chen, Liyi 1 ; Li, Yajuan 1 ; Shao, Guanming 1 ; Zhang, Xinheng 1 ; Wong, Yung Hou 6 ; Xie, Qingmei 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, State Key Lab Swine & Poultry Breeding Ind, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Coll Anim Sci, Heyuan Branch, Guangdong Prov Lab Lingnan Modern Agr Sci & Techno, Guangzhou 510642, Peoples R China
3.Shaoguan Univ, Henry Fok Sch Biol & Agr, Shaoguan 512005, Peoples R China
4.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Peoples R China
5.Guangdong Engn Res Ctr Vector Vaccine Anim Virus, Guangzhou 510642, Peoples R China
6.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China
7.Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Hong Kong, Peoples R China
关键词： circRNA; ALV-J; chicken embryonic stem cell
期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）
ISSN： 0032-5791
年卷期： 2024 年 103 卷 8 期
页码：
收录情况： SCI
摘要： Exosome-mediated horizontal and vertical transmission of subgroup J avian leukosis virus ( ALV-J ) in poultry flocks can lead to growth inhibition and severe immunosuppression. However, there are few reports on the early infection of chicken embryonic stem cells ( cESCs ) with ALV-J. In this study, we con firmed that early infection with ALV-J can accelerate the differentiation of cESCs and promote the secretion of exosomes. To investigate the modulation strategy of ALV-J in cESCs, circRNA sequencing was performed for further analysis. A total of 305 differentially expressed circRNAs ( DECs ) were obtained, including 71 upregulated DECs. Circ-CCDC7 was found to be the most upregulated DEC and was assessed by qRT-PCR, with the result consistent with the result of circRNAseq. Based on qRT-PCR, gga-miR-6568-3p was found to be the target of the top 3 DECs, including circ-CCDC7, and the stem cell marker gene Pax7 was identi fied as the target gene of gga-miR-6568-3p. This study demonstrated that exosomal circ-CCDC7/gga-miR-6568-3p/ Pax7 accelerates the differentiation of cESCs after early infection with ALV-J.
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