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Genome Analysis of a Novel Polysaccharide-Degrading Bacterium Paenibacillus algicola and Determination of Alginate Lyases

文献类型： 外文期刊

作者： Huang, Huiqin 1 ; Zheng, Zhiguo 1 ; Zou, Xiaoxiao 1 ; Wang, Zixu 1 ; Gao, Rong 1 ; Zhu, Jun 1 ; Hu, Yonghua 1 ; Bao, Shixiang 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Inst Trop Agr Resources, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Zhanjiang Expt Stn, Zhanjiang 524013, Peoples R China

3.Hainan Prov Key Lab Funct Components Res & Utiliz, Haikou 571101, Hainan, Peoples R China

4.Hebei Agr Univ, Coll Oceanog, Qinhuangdao 066000, Hebei, Peoples R China

5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

关键词： Paenibacillus algicola; genome; polysaccharide lyase; alginate lyase; oligosaccharide

期刊名称：MARINE DRUGS （ 影响因子：6.085； 五年影响因子：6.044 ）

ISSN：

年卷期： 2022 年 20 卷 6 期

页码：

收录情况： SCI

摘要： Carbohydrate-active enzymes (CAZymes) are an important characteristic of bacteria in marine systems. We herein describe the CAZymes of Paenibacillus algicola HB172198(T), a novel type species isolated from brown algae in Qishui Bay, Hainan, China. The genome of strain HB172198(T) is a 4,475,055 bp circular chromosome with an average GC content of 51.2%. Analysis of the nucleotide sequences of the predicted genes shows that strain HB172198(T) encodes 191 CAZymes. Abundant putative enzymes involved in the degradation of polysaccharides were identified, such as alginate lyase, agarase, carrageenase, xanthanase, xylanase, amylases, cellulase, chitinase, fucosidase and glucanase. Four of the putative polysaccharide lyases from families 7, 15 and 38 were involved in alginate degradation. The alginate lyases of strain HB172198(T) exhibited the maximum activity 152 U/mL at 50 degrees C and pH 8.0, and were relatively stable at pH 7.0 and temperatures lower than 40 degrees C. The average degree of polymerization (DP) of the sodium alginate oligosaccharide (AOS) degraded by the partially purified alginate lyases remained around 14.2, and the thin layer chromatography (TCL) analysis indicated that it contained DP2-DP8 oligosaccharides. The complete genome sequence of P. algicola HB172198(T) will enrich our knowledge of the mechanism of polysaccharide lyase production and provide insights into its potential applications in the degradation of polysaccharides such as alginate.

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