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Promoter of Vegetable Soybean GmTIP1;6 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis

文献类型： 外文期刊

作者： Feng, Zhijuan 1 ; Liu, Na 1 ; Zhang, Guwen 1 ; Bu, Yuanpeng 1 ; Wang, Bin 1 ; Gong, Yaming 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables,Minist Agr & Rural Affairs, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Vegetable Legumes Germplasm Enhancement &, Hangzhou 310021, Peoples R China

关键词： vegetable soybean; aquaporin; promoter; GUS; transgenic Arabidopsis; abiotic stresses; hormone signals; GmTIP1; 6

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2022 年 23 卷 20 期

页码：

收录情况： SCI

摘要： Tonoplast intrinsic proteins (TIPs), a sub-family of aquaporins (AQPs), are known to play important roles in plant abiotic stress responses. However, evidence for the promoters of TIPs involvement in abiotic stress processes remains scarce. In this study, the promoter of the vegetable soybean GmTIP1;6 gene, which had the highest similarity to TIP1-type AQPs from other plants, was cloned. Expression pattern analyses indicated that the GmTIP1;6 gene was dramatically induced by drought, salt, abscisic acid (ABA), and methyl jasmonate (MeJA) stimuli. Promoter analyses revealed that the GmTIP1;6 promoter contained drought, ABA, and MeJA cis-acting elements. Histochemical staining of the GmTIP1;6 promoter in transgenic Arabidopsis corroborated that it was strongly expressed in the vascular bundles of leaves, stems, and roots. Beta-glucuronidase (GUS) activity assays showed that the activities of the GmTIP1;6 promoter were enhanced by different concentrations of polyethylene glycol 6000 (PEG 6000), NaCl, ABA, and MEJA treatments. Integrating these results revealed that the GmTIP1;6 promoter could be applied for improving the tolerance to abiotic stresses of the transgenic plants by promoting the expression of vegetable soybean AQPs.

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