RNA-protein interactions reveals the pivotal role of lncRNA1840 in tomato fruit maturation
文献类型： 外文期刊
作者： Zhu, Guoning 1 ; Li, Ran 1 ; Zhang, Lingling 1 ; Ma, Liqun 1 ; Li, Jinyan 1 ; Chen, Jieyin 2 ; Deng, Zhiping 3 ; Yan, Shijie 4 ; Li, Tao 5 ; Ren, Huazhong 6 ; Cui, Kaicheng 7 ; Qu, Guiqin 1 ; Zhu, Benzhong 1 ; Fu, Daqi 1 ; Luo, Yunbo 1 ; Zhu, Hongliang 1 ;
作者机构： 1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
4.Tianjin Agr Univ, Coll Food Sci & Biol Engn, Tianjin 300392, Peoples R China
5.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China
6.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
7.Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Hubei, Peoples R China
关键词： tomato; Solanum lycopersicum; fruit ripening; long non-coding RNA; ethylene; chromatin isolation by RNA purification
期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）
ISSN： 0960-7412
年卷期： 2024 年 120 卷 2 期
页码：
收录情况： SCI
摘要： Long non-coding RNAs (lncRNAs) play crucial roles in various biological processes in plants. However, the functional mechanism of lncRNAs in fruit ripening, particularly the transition from unripe to ripe stages, remains elusive. One such lncRNA1840, reported by our group, was found to have important role in tomato fruit ripening. In the present study, we gain insight into its functional role in fruit ripening. CRISPR-Cas9 mediated lncRNA1840 mutants caused the delayed tomato fruit ripening. Notably, loss function of lncRNA1840 did not directly impact ethylene signaling but rather delay ethylene synthesis. Transcriptomic analysis revealed differences in the expression of ripening related genes in lncRNA1840 mutants, suggesting that it is involved in gene regulation of fruit ripening. We used Chromatin Isolation by RNA Purification (ChIRP)-Seq to identify lncRNA1840 binding sites on chromatin. ChIRP-seq suggested that lncRNA1840 had occupancy on 40 genes, but none of them is differentially expressed genes in transcriptomic analysis, which indicated lncRNA1840 might indirectly modulate the gene expression. ChIRP-mass spectrometry analysis identified potential protein interactors of lncRNA1840, Pre-mRNA processing splicing factor 8, highlighting its involvement in post-transcriptional regulatory pathways. In summary, lncRNA1840 is key player in tomato plant growth and fruit ripening, with multifaceted roles in gene expression and regulatory networks.
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