Marek's disease virus-encoded MicroRNA-M6-5p suppresses viral replication by targeting viral major capsid protein-coding gene UL19
文献类型： 外文期刊
作者： Zhou, Linyi 1 ; Yang, Jing 1 ; Cheng, Jing 1 ; Liu, Wenxiao 1 ; Wang, Yong 1 ; Li, Yongqing 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China
2.Beijing Univ Agr, Coll Anim Sci & Technol, Beijing 102206, Peoples R China
3.Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing 100097, Peoples R China
4.Sino UK Joint Lab Prevent & Control Infect Dis Liv, Beijing 100097, Peoples R China
关键词： Marek's disease virus; MiRNA; Replication; Viral capsid; UL19
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）
ISSN： 0378-1135
年卷期： 2025 年 307 卷
页码：
收录情况： SCI
摘要： Marek's disease virus (MDV) is a cell-associated alphaherpesvirus that causes lymphoproliferative diseases in chickens. MicroRNAs (miRNAs) are a class of 20-25-nucleotide long single-stranded non-coding RNAs that play an important role in post-transcriptional regulation. MDV-encoded miRNAs are critical for viral replication and tumorigenesis; however, the underlying mechanisms remain unclear. We have previously found that MDVencoded miR-M6-5p inhibits viral replication in vitro. In this study, we identified the major viral capsid protein-coding gene UL19 as a direct functional target of miR-M6-5p. The overexpression of miR-M6-5p significantly reduced UL19 expression, accompanied by a decrease in the number of viral particles. In contrast, the knockout of miR-M6-5p enhanced UL19 expression, accompanied by an increase in the number of viral particles. Consistently, MDV mutants with UL19 deletions could not replicate and assemble capsid structures in infected cells. Thus, miR-M6-5p inhibits MDV replication by impairing capsid assembly by targeting the UL19 gene. These findings reveal how virus-encoded miRNAs regulate viral replication by targeting structural morphogenic components and broaden our understanding of the role of MDV-encoded miRNAs in viral replication.
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