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Isolation and characterization of 66 SNP markers in blotched snakehead (Channa maculata) using 2b-RAD sequencing

文献类型： 外文期刊

作者： Liu, Lan 1 ; Liu, Haiyang 2 ; Xia, Weiwei 1 ; Chen, Kunci 2 ; Luo, Qing 2 ; Ou, Mi 2 ; Zhao, Jian 2 ;

作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Guangzhou 510380, Peoples R China

关键词： Channa maculata; SNP; 2b-RAD; Conservation genetics

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：1.1； 五年影响因子：0.7 ）

ISSN： 1877-7252

年卷期： 2023 年 15 卷 4 期

页码：

收录情况： SCI

摘要： Blotched snakehead (Channa maculata) is an economically important aquaculture species in China and Southeast Asia, but wild blotched snakehead resources have declined sharply in recent years because of its overexploitation and habitat destruction. Thus, it is very urgent to develop genetic markers of blotched snakehead to investigate the natural resources. In this study, a total of 66 novel single nucleotide polymorphism (SNP) markers were developed based on 2b-RAD simplified genome sequencing. The results showed that the observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.130 to 0.667 and 0.112 to 0.502, respectively. The PIC ranged from 0.116 to 0.375. Two loci showed significant deviations from the HWE after Bonferroni correction (p < 0.05). These SNP markers will serve as a valuable tool for population genetic analysis, natural resource conservation and selective breeding of this blotched snakehead.

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