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Supplementation of spermidine enhances the quality of postovulatory aged porcine oocytes

文献类型： 外文期刊

作者： Bai, Jie 1 ; Zhang, Yu 2 ; Li, Na 1 ; Cui, Zhaokang 3 ; Zhang, Hanwen 1 ; Liu, Yiting 1 ; Miao, Yilong 1 ; Sun, Shaochen 1 ; Xiong, Bo 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China

关键词： Spermidine; Postovulatory aging; Oocyte quality; ROS; Apoptosis; Autophagy

期刊名称：CELL COMMUNICATION AND SIGNALING （ 影响因子：8.9； 五年影响因子：9.1 ）

ISSN：

年卷期： 2024 年 22 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundSpermidine (SPD) is an intermediate compound in the polyamine metabolism which takes critical part in a variety of cellular processes. In particular, it has been reported to exert anti-aging effects, suppress the age-related diseases, and extend lifespan across species. However, whether it has the favorable influence on the quality of postovulatory aged oocytes remains elusive.MethodsImmunostaining and fluorescence intensity measurement were used to evaluate the effects of postovulatory aging and SPD supplementation on the oocyte fragmentation, spindle/chromosome structure, actin polymerization, dynamics of cortical granules (CGs) and ovastacin, mitochondrial distribution and function, as well as autophagy levels. In addition, in vitro sperm binding assay and in vitro fertilization (IVF) experiment were applied to assess the impacts of postovulatory aging and SPD supplementation on the sperm binding ability and fertilization capacity of oocytes.ResultsHere, we showed that supplementation of SPD during postovulatory aging could relieve the deterioration of porcine oocytes. Specifically, we found that postovulatory aging impaired the oocyte quality by damaging the morphological integrity of oocytes, maintenance of spindle/chromosome structure, and dynamics of actin cytoskeleton. Postovulatory aging also weakened the sperm binding ability and fertilization capacity of oocytes by compromising the distribution pattern of CGs and their content ovastacin. Notably, supplementation of SPD attenuated these defects in postovulatory aged porcine oocytes via strengthening mitochondrial function, eliminating excessive reactive oxygen species (ROS), inhibiting apoptosis, and enhancing autophagy levels.ConclusionAltogether, our findings demonstrate that SPD supplementation is a feasible approach to ameliorate the quality of postovulatory aged oocytes, which can be potentially applied to the human assisted reproductive technology (ART) and in vitro production of animal embryos.

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