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Comparative analysis of spleen transcriptome of immune response in Sebastes schlegeli against Photobacterium damselae subsp. damselae infection

文献类型： 外文期刊

作者： Yu, Yongxiang 1 ; Wang, Yingeng 1 ; Liao, Meijie 1 ; Shi, Linni 1 ; Li, Yongjie 1 ; Li, Bin 1 ; Rong, Xiaojun 1 ; Wang, C 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Maricultural Organism Dis Control, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

关键词： immune response; Photobacterium damselae subsp; damselae; Sebastes schlegeli; spleen; transcriptome

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期：

页码：

收录情况： SCI

摘要： The previous studies have indicated that Photobacterium damselae subsp. damselae (Pdd) was the pathogen of black rockfish (Sebastes schlegeli) with seriously skin ulcer symptom. This study aimed to explore the spleen transcriptome response of S. schlegeli at 24 h (P24 group) and 48 h (P48 group) after Pdd infection and characterize expression profile of immune-related genes. The fish in the control group were injected with equal volume of PBS. Results showed that there were 2,139 (88.42%), 510 (21.08%) and 242 (10%) differentially expressed genes in PBS vs P24, PBS vs P48 and P24 vs P48 respectively. As the time increases, the number of differential genes gradually decreases. GO annotation indicated that the immune-related function category contributed the most in the PBS vs P24 and PBS vs P48 groups, including immune response, immune system process, immune effector process and regulation of immune system process. KEGG enrichment analysis showed that these genes were located on 277, 184 and 164 signalling pathways in the PBS vs P24, PBS vs P48 and P24 vs P48 groups respectively; among them, 30, 26 and 16 pathways were related to immune system, involving 344, 119 and 24 genes. P24 group involved more immune-related signal pathways and genes than P48 group. RT-qPCR validation indicated that the expression patterns of 10 genes at different time points were well agreed with RNA-Seq analysis. This study provided a better understanding of the molecular responses to photobacteriosis in S. schlegeli.

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