KLF4 promotes milk fat synthesis by regulating the PI3K-AKT-mTOR pathway and targeting FASN activation in bovine mammary epithelial cells
文献类型： 外文期刊
作者： Wu, Hong -Yu 1 ; Ji, Zhong-Hao 1 ; Xie, Wen -Yin 1 ; Guo, Hai -Xiang 1 ; Zheng, Yi 1 ; Gao, Wei 1 ; Yuan, Bao 1 ;
作者机构： 1.Jilin Univ, Coll Anim Sci, Dept Lab Anim, Changchun 130062, Jilin, Peoples R China
2.Changzhi Med Coll, Dept Basic Med, Changzhi 046000, Shanxi, Peoples R China
3.Jilin Acad Agr Sci, Jilin 132101, Peoples R China
期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）
ISSN：
年卷期： 2024 年 27 卷 6 期
页码：
收录情况： SCI
摘要： Milk fat is an important indicator for evaluating the quality of cow's milk. In this study, we used bovine mammary epithelial cells (BMECs) to investigate the role and molecular mechanism of KLF4 in the regulation of milk fat synthesis. The results showed that KLF4 was more highly expressed in mammary tissues of high -fat cows compared with low -fat cows. KLF4 positively regulated the expression of genes related to milk fat synthesis in BMECs, increasing intracellular triglycerides content, and KLF4 promoted milk fat synthesis by activating the PI3K-AKT-mTOR signaling pathway. Furthermore, the results of animal experiments also confirmed that knockdown of KLF4 inhibited milk fat synthesis. In addition, yeast one -hybrid assays and dual-luciferase reporter gene assays confirmed that KLF4 directly targets and binds to the fatty acid synthase (FASN) promoter region to promote FASN transcription. These results demonstrate that KLF4 is a key transcription factor for milk fat synthesis in BMECs.
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