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An electrochemical biosensor for sensitive detection of live Salmonella in food via MXene amplified methylene blue signals and electrostatic immobilization of bacteriophages

文献类型： 外文期刊

作者： Deng, Tingliu 1 ; Wu, Wuming 2 ; Zhou, Jingjing 3 ; Zeng, Qin 1 ; Wang, Heye 5 ; Deng, Chunyan 1 ;

作者机构： 1.Cent South Univ, Coll Chem & Chem Engn, Hunan Prov Key Lab Micro & Nano Mat Interface Sci, Changsha 410083, Peoples R China

2.Hunan Univ Informat Technol, Sch Elect Sci & Engn, Changsha 410151, Peoples R China

3.Capital Med Univ, Beijing Anzhen Hosp, Beijing Key Lab Maternal Fetal Med & Fetal Heart D, Beijing 100069, Peoples R China

4.Capital Med Univ, Beijing Anzhen Hosp, Echocardiog Dept, Beijing 100069, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing 210014, Peoples R China

6.Bright Dairy & Food Co Ltd, Shanghai Engn Res Ctr Dairy Biotechnol, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai 200436, Peoples R China

关键词： Phages; Oriented immobilization; MXene; Nanostructured electrodes; Modified glassy carbon electrode; Differential pulse voltammetry; Salmonella

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：5.0 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 9 期

页码：

收录情况： SCI

摘要： A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization. This allows for a high-density immobilization of bacteriophage particles, achieving approximately 71 pcs mu m(-2). Remarkably, the bacteriophages immobilized MXene nanostructured electrodes still maintain their viability and functionality, ensuring their effectiveness in pathogen detection. Therefore, the proposed biosensor exhibited enhanced sensitivity with a low limit of detection (LOD) of 5 CFU mL(-1). Notably, the biosensor shows excellent specificity in the presence of other bacteria that commonly contaminate food and can distinguish live Salmonella from a mixed population. Furthermore, it is applicable in detecting live Salmonella in food samples, which highlights its potential in food safety monitoring. This biosensor offers simplicity, convenience, and suitability for resource-limited environments, making it a promising tool for on-site monitoring of foodborne pathogenic bacteria.

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