文献类型： 外文期刊

作者： Li, Siyuan ¹ ; Huang, Jin ¹ ; Cai, Xuhang ¹ ; Mao, Li ¹ ; Xie, Lingling ⁷ ; Wang, Fu ⁷ ; Zhou, Hua ⁸ ; Yuan, Xuesong ¹ ; Sun, Xinru ¹ ; Fu, Xincheng ⁹ ; Fan, Baochao ¹ ; Xu, Xingang ³ ; Li, Jizong ¹ ; Li, Bin ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

2.State Key Lab Cultivat Base Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, Key Lab Vet Biol Engn & Technol Minist Agr, Nanjing 210014, Peoples R China

3.Northwest A&F Univ, Coll Vet Med, Xianyang 712100, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

5.Jiangsu Univ, Inst Life Sci, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

6.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

7.Guizhou Testing Ctr Livestock & Poultry Germplasm, Guiyang 550018, Peoples R China

8.Qianxi Anim Dis Control Ctr, Qianxi 551500, Peoples R China

9.Langfang Municipal Bur Agr & Rural Affairs, Langfang 065000, Peoples R China

关键词： bovine coronavirus; cattle; diarrhea; genotypes; genetic evolution

期刊名称：VETERINARY SCIENCES （ 影响因子：2.0； 五年影响因子：2.2 ）

ISSN：

年卷期： 2024 年 11 卷 6 期

页码：

收录情况： SCI

摘要： Simple Summary Bovine coronavirus (BCoV), bovine rotavirus, bovine viral diarrhea virus, and bovine astrovirus are the most common intestinal pathogenic viruses causing diarrhea in cattle. BCoV was the major pathogen detected, with a positive rate of 34.02% (560/1646) from January 2020 to August 2023. The asymptomatic BCoV-infected rate (351/1646) was higher than the diarrhea BCoV-infected rate (209/1646). Studying the relevance of diarrhea associated with BCoV has shown that the onset of diarrheal symptoms post-infection is strongly correlated with the cattle's age and may also be related to the breed. One the other hand, we amplified and sequenced the hemagglutinin esterase (HE), spike protein, and whole genomes of the partially positive samples and obtained six complete HE sequences, seven complete spike sequences, and six whole genomes. The results of molecular characterization revealed that six strains were in the GIIb subgroup, and HBSJZ2202 and JSYZ2209 had four amino acid insertions on HE. We also analyzed ORF1a and found disparities across various regions within GIIb, which were positioned on separate branches within the phylogenetic tree. This work provides data for further investigating the epidemiology of BCoV and for understanding and analyzing BCoV distribution and dynamics. Bovine coronavirus (BCoV), bovine rotavirus, bovine viral diarrhea virus, and bovine astrovirus are the most common intestinal pathogenic viruses causing diarrhea in cattle. We collected 1646 bovine fecal samples from January 2020 to August 2023. BCoV was the major pathogen detected, with a positive rate of 34.02% (560/1646). Of the 670 diarrheal samples and 976 asymptomatic samples, 209 and 351 were BCoV-positive, respectively. Studying the relevance of diarrhea associated with BCoV has shown that the onset of diarrheal symptoms post-infection is strongly correlated with the cattle's age and may also be related to the breed. We amplified and sequenced the hemagglutinin esterase (HE), spike protein, and whole genomes of the partially positive samples and obtained six complete HE sequences, seven complete spike sequences, and six whole genomes. Molecular characterization revealed that six strains were branched Chinese strains, Japanese strains, and partial American strains from the GIIb subgroup. Strains HBSJZ2202 and JSYZ2209 had four amino acid insertions on HE. We also analyzed ORF1a and found disparities across various regions within GIIb, which were positioned on separate branches within the phylogenetic tree. This work provides data for further investigating the epidemiology of BCoV and for understanding and analyzing BCoV distribution and dynamics.