Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro
文献类型： 外文期刊
作者： Gu, Kaiyan 1 ; Zhang, Ya 1 ; Zhong, Ying 1 ; Kan, Yuting 1 ; Jawad, Muhammad 1 ; Gui, Lang 1 ; Ren, Mingchun 4 ; Xu, Gangchun 4 ; Liu, Dong 1 ; Li, Mingyou 1 ;
作者机构： 1.Shanghai Ocean Univ, Key Lab Integrated Rice Fish Farming, Minist Agr & Rural Affairs, Shanghai 201306, Peoples R China
2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Microecol Resources & Utilizat Breeding In, Guangzhou 511400, Peoples R China
4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr, Wuxi 214081, Peoples R China
关键词： Coilia nasus; spermatogonial stem cell line; spermatids; in vitro spermatogenesis; cryopreservation
期刊名称：BIOLOGY-BASEL （ 影响因子：4.2； 五年影响因子：4.4 ）
ISSN：
年卷期： 2023 年 12 卷 9 期
页码：
收录情况： SCI
摘要： The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis. Recapitulation of spermatogenesis in vitro is hindered by the challenge of collecting spermatogonial stem cells under long-term in vitro culture conditions. Coilia nasus is a commercially valuable anadromous migrant fish found in the Yangtze River in China. In the past few decades, exploitation and a deteriorating ecological environment have nearly caused the extinction of C. nasus's natural resources. In the present study, we established a stable spermatogonial stem cell line (CnSSC) from the gonadal tissue of the endangered species C. nasus. The cell line continued to proliferate and maintain stable cell morphology, a normal diploid karyotype, and gene expression patterns after more than one year of cell culture (>80 passages). Additionally, CnSSC cells could successfully differentiate into sperm cells through a coculture system. Therefore, the establishment of endangered species spermatogonial stem cell lines is a model for studying spermatogenesis in vitro and a feasible way to preserve germplasm resources.
- 相关文献
作者其他论文 更多>>
-
Apoptosis, MAPK signaling pathway affected in tilapia liver following nano-microplastics and sulfamethoxazole acute co-exposure
作者：Zheng, Yao;Li, Jiajia;Gao, Jiancao;Hu, Jiawen;Sun, Yi;Zhu, Haojun;Xu, Gangchun;Zheng, Yao;Jin, Wei;Xu, Gangchun
关键词：Liver; Endoplasmic reticulum; MAPK signaling pathway; Acute exposure
-
Effects of Seaweed Polysaccharide on the Growth and Physiological Health of Largemouth Bass, Micropterus salmoides
作者：Huang, Dongyu;Liang, Hualiang;Ren, Mingchun;Gu, Jiaze;Xue, Chunyu
关键词：seaweed polysaccharide; immunity; endoplasmic reticulum stress
-
Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via Bursicon in the Cerebral Ganglion of Chinese Mitten Crab Eriocheir sinensis Under Alkaline Stress
作者：Wang, Meiyao;Xu, Gangchun;Tang, Yongkai;Wang, Meiyao;Xu, Gangchun;Tang, Yongkai;Zhou, Jun;Ge, Jiachun
关键词：
Eriocheir sinensis ; cerebral ganglia; alkalinity stress;bursicon ; signal transduction -
A Study on the Dietary Yeast Polysaccharide Supplementation in Growth Performance, Antioxidant Capacity, and Immunity of Juvenile Largemouth Bass (Micropterus salmoides)
作者：Qin, Junjie;Ren, Mingchun;Liang, Hualiang;Mi, Haifeng;Zhang, Lu;Teng, Tao;Yin, Heng;Ren, Mingchun;Huang, Dongyu;Liang, Hualiang
关键词：juvenile largemouth bass; dietary supplementation; yeast polysaccharide; antioxidant capacity; immunity
-
Polystyrene nanoplastics amplify the toxic effects of PFOA on the Chinese mitten crab (Eriocheir sinensis)
作者：Huang, Peng;Cao, Liping;Du, Jinliang;Guo, Yiqing;Xu, Gangchun;Gao, Jiancao;Cao, Liping;Du, Jinliang;Li, Quanjie;Sun, Yi;Zhu, Haojun;Xu, Gangchun;Gao, Jiancao
关键词：NPs; PFAS; Aquatic crustacean; Multi-omics; Combined toxicity
-
Probiotic Supplementation Improves Lipid Metabolism Disorders and Immune Suppression Induced by High-Fat Diets in Coilia nasus Liver
作者：Gao, Jun;Sun, Yi;Xu, Gangchun;Mang, Qi
关键词：
Coilia nasus ; 4D-DIA proteomics; probiotics supplementation; high-fat diet; lipid metabolism; metabolism disorder -
Microbial-algal symbiotic system drives reconstruction of nitrogen, phosphorus, and methane cycles for purification of pollutants in aquaculture water
作者：Gao, Jun;Mang, Qi;Li, Quanjie;Sun, Yi;Xu, Gangchun;Mang, Qi
关键词：Bacillus subtilis; Chlorella pyrenoidosa; Cross feeding; Environmental microorganisms; Element cycles
