Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro
文献类型： 外文期刊
作者： Gu, Kaiyan 1 ; Zhang, Ya 1 ; Zhong, Ying 1 ; Kan, Yuting 1 ; Jawad, Muhammad 1 ; Gui, Lang 1 ; Ren, Mingchun 4 ; Xu, Gangchun 4 ; Liu, Dong 1 ; Li, Mingyou 1 ;
作者机构： 1.Shanghai Ocean Univ, Key Lab Integrated Rice Fish Farming, Minist Agr & Rural Affairs, Shanghai 201306, Peoples R China
2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Microecol Resources & Utilizat Breeding In, Guangzhou 511400, Peoples R China
4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr, Wuxi 214081, Peoples R China
关键词： Coilia nasus; spermatogonial stem cell line; spermatids; in vitro spermatogenesis; cryopreservation
期刊名称：BIOLOGY-BASEL （ 影响因子：4.2； 五年影响因子：4.4 ）
ISSN：
年卷期： 2023 年 12 卷 9 期
页码：
收录情况： SCI
摘要： The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis. Recapitulation of spermatogenesis in vitro is hindered by the challenge of collecting spermatogonial stem cells under long-term in vitro culture conditions. Coilia nasus is a commercially valuable anadromous migrant fish found in the Yangtze River in China. In the past few decades, exploitation and a deteriorating ecological environment have nearly caused the extinction of C. nasus's natural resources. In the present study, we established a stable spermatogonial stem cell line (CnSSC) from the gonadal tissue of the endangered species C. nasus. The cell line continued to proliferate and maintain stable cell morphology, a normal diploid karyotype, and gene expression patterns after more than one year of cell culture (>80 passages). Additionally, CnSSC cells could successfully differentiate into sperm cells through a coculture system. Therefore, the establishment of endangered species spermatogonial stem cell lines is a model for studying spermatogenesis in vitro and a feasible way to preserve germplasm resources.
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