文献类型： 外文期刊

作者： Du, Jinliang ¹ ; Cao, Liping ¹ ; Gao, Jiancao ² ; Nie, Zhijuan ¹ ; Li, Quanjie ² ; Sun, Yi ² ; Shao, Nailin ² ; Hu, Jiawen ² ; Zhou, Lin ² ; Yin, Guojun ¹ ; Xu, Gangchun ¹ ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr & Rural Affairs, Key Lab Freshwater Fisheries & Germplasm Resources, Wuxi 214081, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Int Joint Res Lab Fish Immunopharmacol, Wuxi 214081, Peoples R China

4.Nanjing Agr Univ, Chinese Acad Fishery Sci, Wuxi Fisheries Coll, Freshwater Fisheries Res Ctr,Key Lab Freshwater Fi, Wuxi 214081, Peoples R China

关键词： Microcystin-LR (MC-LR); Chinese mitten crab ( Eriocheir sinensis ); Antioxidant enzymes; Intestinal microbiome

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2024 年 35 卷

页码：

收录情况： SCI

摘要： To investigate the potential toxic mechanism of Microcystin-LR (MC-LR) on juvenile Eriocheir sinensis (E. sinensis), a toxicity test was conducted by injecting MC-LR into the 3rd pereiopod. Following injection, we evaluated alterations in immune response biomarkers, antioxidant status, lipid metabolism-related genes expression, and intestinal microbiota composition in the hepatopancreas and intestinal tissues of E. sinensis. The results of immune response showed that after injection of MC-LR, the serum complement C3 level in E. sinensis significantly decreased at 48 hours (P < 0.05), whereas complement C4 level increased considerably from 12 to 96 hours (P < 0.01). The phenoloxidase system (PO) showed an increasing trend from 6 to 96 hours (P < 0.05 or P < 0.01). The levels of TNF-alpha, IL -6 significantly increased at 6 and 12 hours (P < 0.05 or P < 0.01). Gene expression analysis showed that FASN gene in the hepatopancreatic tissue displayed a higher expression at both 6 and 12 hours postexposure (P < 0.01), while ALDH and ACAA2 gene exhibited a consistent downward trend from 6 to 48 hours (P < 0.05), Additionally, the mRNA levels of MECR and ACSL demonstrated a pattern of initial downregulation, followed by an upregulation, and subsequent downregulation. The antioxidant status results revealed that the levels GSH and CAT increased first and then decreased, while the activity of SOD decreased first and then increased. Meanwhile, the MDA content showed an increasing trend. 16 S rDNA analysis showed that the intestinal microbial diversity in MC-LR treatment group was significantly higher relative to the control group (P < 0.01). Compared with the control group, the MC-LR group had a significantly higher relative abundance of Proteobacteria, Fusobacteria, and Actinobacteria (P < 0.05) and a significantly lower relative abundance of Firmicutes (P < 0.01). At genus level, the relative abundance of Acinnetobacter , Flavobacterium , Acidovorax , Paracoccus , Cloacibacterium , Gemmobacter , Aeromonas , and Dechloromonas in the intestine of MC-LR-treated group was significantly increased relative to the control group (P < 0.05 or P < 0.01), but the relative abundance of Lachnospiraceae_NK4A136_group was significantly decreased (P < 0.01). The results of the study demonstrated that MC-LR could induced oxidative stress, hepatopancreas tissue damage, and intestinal microbial imbalance in E. sinensis.