A full-length transcriptome and gene expression analysis of three detoxification gene families in a predatory stink bug, Picromerus lewisi
文献类型： 外文期刊
作者： Li, Wenhong 1 ; Wang, Xinyi 2 ; Jiang, Po 2 ; Yang, Mingwei 2 ; Li, Zhimo 3 ; Huang, Chunyang 3 ; He, Yueping 2 ;
作者机构： 1.Inst Plant Protect, Guizhou Acad Agr Sci, Guiyang, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pest, Wuhan, Peoples R China
3.Guizhou Prov Tobacco Co, Zunyi Branch, Zunyi, Peoples R China
关键词： Picromerus lewisi; predatory stink bug; iso-seq; cytochrome P450 monooxygenase; glutathione S-transferase; carboxylesterase
期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.755； 五年影响因子：5.316 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： The predatory stink bug P. Lewisi shows potential for Integrated Pest Management programs for controlling Lepidoptera pest insects in crops and forests. The importance of this insect for biological control has stimulated several studies into its biology and ecology. However, P. lewisi has little genetic information available. In the present study, PacBio single-molecule real-time (SMRT) sequencing and Illumina RNA-seq sequencing technologies were used to reveal the full-length transcriptome profiling and tissue-specific expression patterns of P. lewisi. A total of 12,997 high-quality transcripts with an average length of 2,292 bp were obtained from different stages of P. lewisi using SMRT sequencing. Among these, 12,101 were successfully annotated in seven public databases. A total of 67 genes of cytochrome P450 monooxygenases, 43 carboxylesterase genes, and 18 glutathione S-transferase genes were identified, most of which were obtained with full-length ORFs. Then, tissue-specific expression patterns of 5th instar nymphs were analyzed using Illumina sequencing. Several candidate genes related to detoxification of insecticides and other xenobiotics as well as the degradation of odors, were identified in the guts and antennae of P. lewisi. The current study offered in-depth knowledge to understand the biology and ecology of this beneficial predator and related species.
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