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Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos

文献类型： 外文期刊

作者： Wang, Xinyi 1 ; Li, Wenhong 3 ; Yang, Xiang 2 ; Yang, Mingwei 1 ; Gu, Yucheng 1 ; Du, Zhao 1 ; Yang, Jingyi 1 ; Wen, Mingxia 2 ; Park, Yoonseong 4 ; Huang, Chunyang 2 ; He, Yueping 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pest, Wuhan 430070, Peoples R China

2.Guizhou Prov Tobacco Co, Zunyi Branch, Zunyi 56300, Peoples R China

3.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China

4.Kansas State Univ, Dept Entomol, Manhattan, KS USA

5.Huazhong Agr Univ, Natl Engn Res Ctr Microbial Pesticides, Wuhan, Peoples R China

关键词： predatory stink bug; Arma custos; toxin; recombinant protein; insecticidal; Spodoptera litura; Nilaparvata lugens

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUND: Widespread resistance of insect pests to insecticides and transgenic crops in the field is a significant challenge for sustainable agriculture, and calls for the development of novel alternative strategies to control insect pests. One potential resource for the discovery of novel insecticidal molecules is natural toxins, particularly those derived from the venoms of insect predators. RESULTS: In this study, we identified three insecticidal proteinaceous toxins from the venom glands (VGs) of the predatory stink bug, Arma custos (Hemiptera: Asopinae). Transcriptomic analysis of A. custos VGs revealed 151 potentially secreted VG-rich venom proteins. Three VG-rich venom proteins (designated AcVP1 similar to 3) were produced by overexpression in Escherichia coli. Injection of the recombinant proteins into tobacco cutworm (Spodoptera litura) larvae showed that all of the three recombinant proteins caused paralysis, liquefaction and death. Injection of recombinant proteins into rice brown planthopper (Nilaparvata lugens) nymphs showed higher insecticidal activities, among which a trypsin (AcVP2) caused 100% mortality postinjection at 1.27 pmol mg(-1) body weight. CONCLUSION: A natural toolkit for the discovery of insecticidal toxins from predatory insects has been revealed by the present study. (c) 2024 Society of Chemical Industry.

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