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The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops

文献类型： 外文期刊

作者： Wang, Jinbin 1 ; Wang, Yu 1 ; Hu, Xiuwen 1 ; Yang, Qianwen 1 ; Chen, Yifan 1 ; Jiang, Wei 1 ; Liu, Xiaofeng 1 ; Liu, Hua 1 ; Zeng, Haijuan 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Biotechnol Res, Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China

2.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspection & Supervis Ct, 2901 Beidi Rd, Shanghai 201106, Peoples R China

3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China

关键词： Genetically modified crops; Lateral flow test strip; CRISPR; Cas12a; On-site detection

期刊名称：FOOD CONTROL （ 影响因子：6.652； 五年影响因子：6.498 ）

ISSN： 0956-7135

年卷期： 2022 年 139 卷

页码：

收录情况： SCI

摘要： Rapid detection method with high sensitivity has been expected in transgenic field screening. In this study, an ultra-sensitive visualization detection procedure based on recombinase polymerase amplification, CRISPR/ Cas12a system, and lateral flow strip technology (RPA-Cas12a-LFB) was developed for the rapid detection of genetically modified (GM) crops. P-CaMV 35 S and T -NOS screening elements, the most widely covered in GM crops, were targeted. The results showed that standard plasmids as low as 10 copies and 0.01% GM crops of Bt11 and MON863 samples can be detected, demonstrating the ultra-sensitivity of the method. The assay was applied to a series of GM crop standards, and the results were consistent with those of existing methods, exhibiting the excellent specificity and feasibility. The analysis can be completed within 40 min, realizing rapid detection. Overall, an accurate, sensitive, time-saving, and instrument-independent detection system was successfully developed, providing a new strategy with ultra-sensitive capability in detection.

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