您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The Development of a Procedure for the Cryopreservation of the Callus of Anthurium andraeanum by Vitrification

文献类型： 外文期刊

作者： Zhang, Yiying 1 ; Deng, Shan 1 ; Lin, Huifeng 2 ; Chu, Yunxia 1 ; Huang, Jingyan 1 ; Li, Shouguo 1 ; Lin, Fazhuang 2 ; Zhang, Sumei 3 ; Jiang, Weilan 4 ; Ren, Li 1 ; Chen, Hairong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, 888 Rd Yezhuang, Shanghai 201403, Peoples R China

2.Sanming Acad Agr Scienses, Flower Res Inst, 2 Str Qiujiang Zhuyuan, Sanming 365051, Shaxian, Peoples R China

3.Yangzhou Univ, Coll Biosci & Biotechnol, 48 Rd Wenhui, Yangzhou 225009, Peoples R China

4.Anshun Univ, Sch Agr, 25 Rd Xueyuan, Anshun 561000, Peoples R China

关键词： Anthurium andraeanum; callus culture; cryopreservation; vitrification

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 21 期

页码：

收录情况： SCI

摘要： The cryopreservation of Anthurium andraeanum germplasm resources is extremely important for the production and selection of new varieties. At present, the cryopreservation procedure for the callus of A. andraeanum has not been established. In this study, the leaves of A. andraeanum were used as explants to culture the callus. The cryopreservation procedure of the callus by vitrification was initially established by using the orthogonal experimental method of four factors and three levels in the preculture, loading, and dehydration steps. Furthermore, the vitrification-based cryopreservation was optimized by changing the preculture temperature and loading solution and adding exogenous substances to the plant vitrification solution (PVS2). In this procedure, the callus was precultured at 25 degrees C for 2 d, and loaded in 50% PVS2 at 25 degrees C for 60 min. The callus was dehydrated with PVS2 containing 0.08 mM reduced glutathione (GSH) at 0 degrees C for 60 min. After rapid-cooling in liquid nitrogen for 1 h, it was rapid-warming in a water bath at 40 degrees C for 90 s and unloaded for 30 min. After 1 d of recovery, the cell relative survival rate of the cryopreserved callus was 64.60%. The results provide a valuable basic and effective method for the long-term conservation of A. andraeanum germplasm resources.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台