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PollenDetect: An Open-Source Pollen Viability Status Recognition System Based on Deep Learning Neural Networks

文献类型： 外文期刊

作者： Tan, Zhihao 1 ; Yang, Jing 1 ; Li, Qingyuan 3 ; Su, Fengxiang 1 ; Yang, Tianxu 1 ; Wang, Weiran 2 ; Aierxi, Alifu 2 ; Zhang, Xianlong 1 ; Yang, Wanneng 1 ; Kong, Jie 2 ; Min, Ling 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Econ Crops, Urumqi 830091, Peoples R China

3.Wuhan Acad Agr Sci, Forestry & Fruit Tree Res Inst, Wuhan 430075, Peoples R China

关键词： computer vision; deep learning; high temperature stress; open-source; pollen viability

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 21 期

页码：

收录情况： SCI

摘要： Pollen grains, the male gametophytes for reproduction in higher plants, are vulnerable to various stresses that lead to loss of viability and eventually crop yield. A conventional method for assessing pollen viability is manual counting after staining, which is laborious and hinders high-throughput screening. We developed an automatic detection tool (PollenDetect) to distinguish viable and nonviable pollen based on the YOLOv5 neural network, which is adjusted to adapt to the small target detection task. Compared with manual work, PollenDetect significantly reduced detection time (from approximately 3 min to 1 s for each image). Meanwhile, PollenDetect can maintain high detection accuracy. When PollenDetect was tested on cotton pollen viability, 99% accuracy was achieved. Furthermore, the results obtained using PollenDetect show that high temperature weakened cotton pollen viability, which is highly similar to the pollen viability results obtained using 2,3,5-triphenyltetrazolium formazan quantification. PollenDetect is an open-source software that can be further trained to count different types of pollen for research purposes. Thus, PollenDetect is a rapid and accurate system for recognizing pollen viability status, and is important for screening stress-resistant crop varieties for the identification of pollen viability and stress resistance genes during genetic breeding research.

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