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RAPID LEAF FALLING 1 facilitates chemical defoliation and mechanical harvesting in cotton

文献类型： 外文期刊

作者： Zhang, Bing 1 ; Yue, Dandan 1 ; Han, Bei 1 ; Bao, Danfan 1 ; Zhang, Xiao 1 ; Hao, Xuyang 1 ; Lindsey, Keith 2 ; Lin, Xin 1 ; Zhu, Longfu 1 ; Jin, Shuangxia 1 ; Wang, Maojun 1 ; Xu, Haijiang 3 ; Du, Mingwei 4 ; Yu, Yu 5 ; Zhang, Xianlong 1 ; Yang, Xiyan 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Univ Durham, Dept Biosci, South Rd, Durham DH1 3LE, England

3.Xinjiang Acad Agr Sci, Inst Econ Crops, Urumqi 830091, Xinjiang, Peoples R China

4.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China

5.Xinjiang Acad Agr & Reclamat Sci, Cotton Inst, Shihezi 832000, Xinjiang, Peoples R China

关键词： abscission cells; chemical defoliation; snRNA-seq; machine-harvested cotton; RAPID LEAF FALLING 1

期刊名称：MOLECULAR PLANT （ 影响因子：24.1； 五年影响因子：25.8 ）

ISSN： 1674-2052

年卷期： 2025 年 18 卷 5 期

页码：

收录情况： SCI

摘要： Chemical defoliation stands as the ultimate tool in enabling the mechanical harvest of cotton, offering economic and environmental advantages. However, the underlying molecular mechanism that triggers leaf abscission through defoliant remains unsolved. In this study, we meticulously constructed a transcriptomic atlas through single-nucleus mRNA sequencing (snRNA-seq) of the abscission zone (AZ) from cotton petiole. We identified two newly-formed cell types, abscission cells and protection layer cells in cotton petiole AZ after defoliant treatment. GhRLF1 (RAPID LEAF FALLING 1), as one of the members of the cytokinin oxidase/dehydrogenase (CKX) gene family, was further characterized as a key marker gene unique to the abscission cells following defoliant treatment. Overexpression of GhRLF1 resulted in reduced cytokinin accumulation and accelerated leaf abscission. Conversely, CRISPR/Cas9-mediated loss of GhRLF1 function appeared to delay this process. Its interacting regulators, GhWRKY70, acting as "Pioneer"activator, and GhMYB108, acting as "Successor"activator, orchestrate a sequential modulation of GhWRKY70/ GhMYB108-GhRLF1-CTK (cytokinin) within the AZ to regulate cotton leaf abscission. GhRLF1 not only regulates leaf abscission but also reduces cotton yield. Consequently, transgenic lines that exhibit rapid leaf falling and require less defoliant but show unaffected cotton yield were developed for mechanical harvesting. This was achieved using a defoliant-induced petiole-specific promoter, proPER21, to drive GhRLF1 (proPER21::RLF1). This pioneering biotechnology offers a new strategy for the chemical defoliation of machine-harvested cotton, ensuring stable production and reducing leaf debris in harvested cotton, thereby enhancing environmental sustainability.

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