您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect- resistance genes

文献类型： 外文期刊

作者： Wang, Fuqiu 1 ; Liang, Sijia 2 ; Wang, Guanying 1 ; Hu, Tianyu 1 ; Fu, Chunyang 1 ; Wang, Qiongqiong 1 ; Xu, Zhongping 1 ; Fan, Yibo 1 ; Che, Lianlian 1 ; Min, Ling 1 ; Li, Bo 3 ; Long, Lu 4 ; Gao, Wei 4 ; Zhang, Xianlong 1 ; Jin, Shuangxia 1 ;

作者机构： 1.Huazhong Agr Univ, Hubei Hongshan Lab, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Huanghuai Univ, Acad Ind Innovat & Dev, Zhumadian 463000, Henan, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Xinjiang Key Lab Crop Biotechnol, Urumqi 830091, Xinjiang, Peoples R China

4.Henan Univ, Sch Life Sci, Natl Key Lab Cotton Biobreeding & Integrated Utili, Kaifeng 475004, Henan, Peoples R China

关键词： cotton; CDPKs; mutant library; CRISPR-Cas9; Ca 2+influx; insect resistance

期刊名称：PLANT COMMUNICATIONS （ 影响因子：11.6； 五年影响因子：11.8 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 11 期

页码：

收录情况： SCI

摘要： Calcium-dependent protein kinases (CDPKs) act as key signal transduction enzymes in plants, especially in response to diverse stresses, including herbivory. In this study, a comprehensive analysis of the CDPK gene family in upland cotton revealed that GhCPKs are widely expressed in multiple cotton tissues and respond positively to various biotic and abiotic stresses. We developed a strategy for screening insect- resistance genes from a CRISPR-Cas9 mutant library of GhCPKs. The library was created using 246 single-guide RNAs targeting the GhCPK gene family to generate 518 independent T0 plants. The average target-gene coverage was 86.18%, the genome editing rate was 89.49%, and the editing heritability was 82%. An insect bioassay in the field led to identification of 14 GhCPK mutants that are resistant or susceptible to insects. The mutant that showed the clearest insect resistance, cpk33/74 (in which the homologous genes GhCPK33 and GhCPK74 were knocked out), was selected for further study. Oral secretions from Spodoptera litura induced a rapid influx of Ca2+ in cpk33/74 leaves, resulting in a significant increase in jasmonic acid content. S-adenosylmethionine synthase is an important protein involved in plant stress response, and protein interaction experiments provided evidence for interactions of GhCPK33 and GhCPK74 with GhSAMS1 and GhSAM2. In addition, virus-induced gene silencing of GhSAMS1 and GhSAM2 in cotton impaired defense against S. litura. This study demonstrates an effective strategy for constructing a mutant library of a gene family in a polyploid plant species and offers valuable insights into the role of CDPKs in the interaction between plants and herbivorous insects.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台