Preparation, characterization, and potential application of shark derived single domain antibody against myofibrillar binding serine protease in Silver carp
文献类型： 外文期刊
作者： Qu, Yuekuan 1 ; Han, Xiangning 1 ; Liu, Xin 1 ; Wang, Zheng 1 ; Cao, Limin 1 ; Lin, Hong 1 ; Pavase, Tushar Ramesh 1 ; Chen, Shengjun 2 ; Sui, Jianxin 1 ;
作者机构： 1.Ocean Univ China, Food Safety Lab, 5 Yushan Rd, Qingdao 266003, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
期刊名称：JOURNAL OF FOOD PROCESSING AND PRESERVATION （ 影响因子：2.609； 五年影响因子：2.61 ）
ISSN： 0145-8892
年卷期：
页码：
收录情况： SCI
摘要： Myofibrillar binding serine protease (MBSP) existing in fish muscle is the leading cause of gel deterioration, which adversely affects the quality of surimi produces. Screening and preparation of protease inhibitors with high efficiency and specificity is an important approach to address the reduction of surimi quality. In this study, shark derived single domain antibody specific to MBSP was expressed and its properties were investigated systematically. The results showed that single domain antibody specific to MBSP could be expressed in soluble using SUMO fusion tagged Escherichia coli expression system with good thermal and pH stability. The enzyme activity inhibition effect in vitro could reach 62.9% at the concentration of 0.1 mg/ml of single domain antibody. When added in silver carp surimi, it could significantly inhibit the deterioration of surimi gel. The results of this study implied that shark derived single domain antibody could be developed as a new inhibitor of MBSP used for the control of gel degradation in surimi produces. Novelty impact statement Shark derived single domain antibody specific to MBSP was expressed in soluble with high yield and stability; the expressed antibody could significantly inhibit the activity of MBSP both in vitro and in surimi; this good characteristic makes it to be an inhibitor for the deterioration of surimi gel.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification, Sequence Alignment, and Transcription of Five Sex-Related Genes in Largemouth Bass (Micropterus Salmoides)
作者：Zhang, Xinhui;You, Xinxin;Liu, Huan;Liu, Xin;Shi, Qiong;Zhang, Xinhui;Ruan, Zhiqiang;Huang, Yu;You, Xinxin;Wang, Xinwen;Xu, Junmin;Shi, Qiong;Sun, Chengfei;Ye, Xing;Hu, Cancan;Wang, Xinwen;Xu, Junmin;Liu, Huan;Liu, Xin;Shi, Qiong
关键词：largemouth bass; sex-related gene; transcriptome sequencing; gonadal development; differentially expressed genes
-
Novel insight into the formation and improvement mechanism of physical property in fermented tilapia sausage by cooperative fermentation of newly isolated lactic acid bacteria based on microbial contribution
作者：Cui, Qiaoyan;Yang, Yanping;Cui, Qiaoyan;Li, Laihao;Huang, Hui;Chen, Shengjun;Li, Chunsheng;Yang, Yanping
关键词：Tilapia sausage; Cooperative fermentation; Gel strength; Texture
-
In-depth discovery and taste presentation mechanism studies on umami peptides derived from fermented sea bass based on peptidomics and machine learning
作者：Wang, Chunxin;Wu, Yanyan;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Wang, Di;Wang, Yueqi;Wu, Yanyan;Cai, Qiuxing;Wang, Yueqi;Wu, Yanyan;Cai, Qiuxing;Wang, Yueqi;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Wang, Di;Wang, Yueqi;Wang, Chunxin;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Wang, Di;Wang, Yueqi
关键词：Fermented sea bass; Machine learning; Umami peptides; Molecular docking
-
Exploring molecular mechanisms underlying changes in lipid fingerprinting of salmon (Salmo salar) during air frying integrating machine learning-guided REIMS and lipidomics analysis
作者：Song, Gongshuai;Zeng, Mingwei;Jiang, Nengliang;Wang, Danli;Yuan, Tinglan;Li, Ling;Gong, Jinyan;Song, Gongshuai;Chen, Shengjun;Song, Gongshuai;Shen, Qing;Lyu, Zhangfan;Mei, Guangming
关键词：Salmon; Air frying; Lipids oxidation; Lipidomics; Machine learning
-
Brooding fathers actively consume viable embryos to take up nutrients in a monogamous syngnathid fish
作者：Lin, Tingting;Liu, Xin;Li, Siping;Zhang, Dong;Shen, Fengyuan;Lin, Tingting;Liu, Xin;Li, Siping;Zhang, Dong
关键词：filial cannibalism; infanticide; monogamous syngnathid fishes; nurse eggs; seahorse Hippocampus erectus
-
Effect of ozone oxidation on gastrointestinal digestion and absorption characteristics of silver carp ( Hypophthalmichthys molitrix) ) surimi gels in vitro
作者：Cao, Shuning;Pan, Yuping;Zheng, Wendong;Yin, Tao;Liu, Ru;You, Juan;Chen, Shengjun;Yin, Tao;Liu, Ru;You, Juan
关键词：Ozone oxidation; Surimi gels; Digestion and absorption; LC-MS/MS
-
Insights into microbiota community dynamics and flavor development mechanism during golden pomfret (Trachinotus ovatus) fermentation based on single-molecule real-time sequencing and molecular networking analysis
作者：Wang, Yueqi;Chen, Qian;Xiang, Huan;Sun-Waterhouse, Dongxiao;Chen, Shengjun;Zhao, Yongqiang;Li, Laihao;Wu, Yanyan;Wang, Yueqi;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Li, Laihao;Wu, Yanyan;Sun-Waterhouse, Dongxiao;Wang, Yueqi;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Li, Laihao;Wu, Yanyan
关键词：Fermented golden pomfret; Microbiota community; Volatile compound; Co-occurrence network; Metabolic pathway
